Synthesis of [4S- H]NADH, [4R- H]NADH, [4- H ]NADH and [4- H]NAD cofactors through heterogeneous biocatalysis in heavy water.

This practitioner protocol describes the synthesis of a family of deuterated nicotinamide cofactors: [4S- H]NADH, [4R- H]NADH, [4- H ]NADH and [4- H]NAD . The application of a recently developed H -driven heterogeneous biocatalyst enables the cofactors to be prepared with high (>90%) H-incorporation with H O as the only isotope source.