Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland.
Department of Genetics, Plant Breeding and Seed Production, Faculty of Life Sciences and Technology, Wroclaw University of Environmental and Plant Sciences, Wroclaw, Poland.
PLoS One. 2021 Jan 26;16(1):e0246052. doi: 10.1371/journal.pone.0246052. eCollection 2021.
In this study transcriptome was analyzed on two fibrous varieties of flax: the susceptible Regina and the resistant Nike. The experiment was carried out on 2-week-old seedlings, because in this phase of development flax is the most susceptible to infection. We analyzed the whole seedlings, which allowed us to recognize the systemic response of the plants to the infection. We decided to analyze two time points: 24h and 48h, because our goal was to learn the mechanisms activated in the initial stages of infection, these points were selected based on the previous analysis of chitinase gene expression, whose increase in time of Fusarium oxysporum lini infection has been repeatedly confirmed both in the case of flax and other plant species. The results show that although qualitatively the responses of the two varieties are similar, it is the degree of the response that plays the role in the differences of their resistance to F. oxysporum.
在这项研究中，对亚麻的两种纤维品种进行了转录组分析：易感的 Regina 和抗性的 Nike。实验是在 2 周龄的幼苗上进行的，因为在这个发育阶段，亚麻最容易感染。我们分析了整个幼苗，这使我们能够识别植物对感染的系统反应。我们决定分析两个时间点：24 小时和 48 小时，因为我们的目标是了解感染初期激活的机制，这些时间点是基于先前对几丁质酶基因表达的分析选定的，在亚麻和其他植物物种中，已经反复证实了在时间 F. oxysporum lini 感染过程中该基因表达的增加。结果表明，尽管两种品种的反应在定性上相似，但反应的程度在它们对 F. oxysporum 的抗性差异中起着作用。
