School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, People's Republic of China.
School of Pharmaceutical Sciences and Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250014, People's Republic of China.
Int J Nanomedicine. 2022 Apr 6;17:1647-1657. doi: 10.2147/IJN.S356919. eCollection 2022.
The leaves of (LLJ) are widely used as medicine in China. It is rich in caffeoylquinic acids, flavonoids and iridoid glycosides and has strong reducing capacities. Therefore, it can be used as a green material to synthesize silver nanoparticles.
LLJ was used as a reducing agent to produce the LLJ-mediated silver nanoparticles (LLJ-AgNPs). The structure and physicochemical properties of LLJ-AgNPs were characterized by ultraviolet spectroscopy (UV-Vis), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and x-ray powder diffraction (XRD). Antioxidant activity of LLJ-AgNPs was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging. Antibacterial activity was determined by 96 well plates (AGAR) gradient dilution, while the anticancer potential was determined by MTT assay.
The results showed LLJ-AgNPs had a spherical structure with the maximum UV-Vis absorption at 400 nm. In addition, LLJ-AgNPs exhibited excellent antioxidant properties, where the free radical scavenging rate of LLJ-AgNPs was increased from 39% to 92% at concentrations from 0.25 to 1.0 mg/mL. Moreover, LLJ-AgNPs displayed excellent antibacterial properties against and at room temperature, with minimum inhibitory values of 10 and 10 g/L, respectively. In addition, the synthetic LLJ-AgNPs exhibited a better inhibition effect in the proliferation of cancer cells (HepG2, MDA-MB -231, and Hela cells).
The present study provides a green approach to synthesize LLJ-AgNPs. All those findings illustrated that the produced LLJ-AgNPs can be used as an economical and efficient functional material for further applications in food and pharmaceutical fields.
（LLJ）的叶子在中国被广泛用作药物。它富含咖啡酰奎宁酸、类黄酮和环烯醚萜苷，具有很强的还原能力。因此，它可以用作合成银纳米粒子的绿色材料。
以 LLJ 为还原剂制备 LLJ 介导的银纳米粒子（LLJ-AgNPs）。采用紫外可见光谱（UV-Vis）、扫描电子显微镜（SEM）、透射电子显微镜（TEM）、傅里叶变换红外光谱（FTIR）和 X 射线粉末衍射（XRD）对 LLJ-AgNPs 的结构和物理化学性质进行了表征。采用 1,1-二苯基-2-苦基肼（DPPH）清除法测定 LLJ-AgNPs 的抗氧化活性。采用 96 孔板（AGAR）梯度稀释法测定抗菌活性，采用 MTT 法测定抗癌潜力。
结果表明，LLJ-AgNPs 具有球形结构，最大紫外-可见吸收峰在 400nm 处。此外，LLJ-AgNPs 表现出优异的抗氧化性能，其自由基清除率从 0.25 至 1.0mg/mL 浓度范围内从 39%增加到 92%。此外，LLJ-AgNPs 在室温下对 和 表现出优异的抗菌性能，最低抑菌浓度分别为 10 和 10g/L。此外，合成的 LLJ-AgNPs 对癌细胞（HepG2、MDA-MB-231 和 Hela 细胞）的增殖具有更好的抑制作用。
本研究提供了一种绿色合成 LLJ-AgNPs 的方法。所有这些发现表明，所制备的 LLJ-AgNPs 可作为一种经济高效的功能材料，进一步应用于食品和制药领域。
