Young Researchers and Elite Club, Saveh Branch, Islamic Azad University, Saveh, Iran.
Department of Microbiology, Iran University of Medical Sciences, Tehran, Iran.
Int J Nanomedicine. 2020 Sep 21;15:6905-6916. doi: 10.2147/IJN.S260520. eCollection 2020.
The extensive drug-resistant (XDR) () causes a range of infections with high mortality rate, which inflicts additional costs on treatment. The use of nano-biotechnology-based methods in medicine has opened a new perspective against drug-resistant bacteria. The aim of this study was to evaluate the effectiveness of the AgNO3 nanoparticles alone and conjugated with imipenem (IMI) to combat extensively drug-resistant .
Antibiotic susceptibility was carried out using disc diffusion method. Detection of different resistant genes was performed using standard polymerase chain reaction (PCR). The chemically synthesized AgNO particles were characterized using scanning electron microscope (SEM), dynamic light scattering (DLS) and X-ray diffraction (XRD) methods. Fourier transform infrared spectroscopy (FTIR) was accomplished to confirm the binding of AgNO with IMI. The microdilution broth method was used to obtain minimum inhibitory concentration (MIC) of AgNO and IMI-conjugated AgNO. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was carried out on L929 cell line to study the cytotoxicity of nanoparticles. The data were analyzed by Eta correlation ratio and chi-square ( ) test.
Analysis of the antibiotic resistance pattern showed that 12 (24%) isolates were XDR, and MIC values of IMI were between 64 and 128 μg/mL. Frequency of SHV, TEM, CTX M, IMP, VIM, OPR, SIM, SPM, GIM, NDM, VEB, PER, KPC, OXA, intI, intII, and intIII genes were 29 (58%), 26 (52%), 26 (52%), 32 (64%), 23 (46%), 43 (86%), 3 (6%), 6 (12%), 3 (6%), 4 (8%), 7 (14%), 6 (12%), 18 (36%), 4 (8%), 19 (38%), 16 (32%), and 2 (4%), respectively. The XRD, SEM, DLS, and FTIR analysis confirmed the synthesis of AgNO nanoparticles and their conjugation with IMI. The AgNO nanoparticles had antimicrobial activity, and their conjugation with IMI showed enhanced effectiveness against XDR isolates. The synthesized AgNO showed no cytotoxic effects.
The results suggest that IMI-conjugated AgNO has a strong potency as a powerful antibacterial agent against XDR .
广泛耐药（XDR） （）引起的一系列感染具有很高的死亡率，这给治疗带来了额外的成本。基于纳米生物技术的方法在医学中的应用为对抗耐药菌开辟了新的视角。本研究旨在评估单独使用 AgNO3 纳米颗粒和与亚胺培南（IMI）结合对抗广泛耐药 的效果。
使用圆盘扩散法进行抗生素敏感性检测。使用标准聚合酶链反应（PCR）检测不同耐药基因。使用扫描电子显微镜（SEM）、动态光散射（DLS）和 X 射线衍射（XRD）方法对化学合成的 AgNO 颗粒进行表征。傅里叶变换红外光谱（FTIR）用于确认 AgNO 与 IMI 的结合。采用微量稀释肉汤法测定 AgNO 和 AgNO-IMI 偶联物的最小抑菌浓度（MIC）。采用 MTT（3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴盐）法测定 L929 细胞系中纳米颗粒的细胞毒性。采用 Eta 相关比和卡方（ ）检验对数据进行分析。
抗生素耐药模式分析显示，12 株（24%）分离株为 XDR，IMI 的 MIC 值在 64 至 128 μg/mL 之间。SHV、TEM、CTX-M、IMP、VIM、OPR、SIM、SPM、GIM、NDM、VEB、PER、KPC、OXA、intI、intII 和 intIII 基因的频率分别为 29（58%）、26（52%）、26（52%）、32（64%）、23（46%）、43（86%）、3（6%）、6（12%）、3（6%）、4（8%）、7（14%）、6（12%）、18（36%）、4（8%）、19（38%）、16（32%）和 2（4%）。XRD、SEM、DLS 和 FTIR 分析证实了 AgNO 纳米颗粒的合成及其与 IMI 的偶联。AgNO 纳米颗粒具有抗菌活性，与 IMI 偶联可增强其对 XDR 分离株的有效性。合成的 AgNO 无细胞毒性。
结果表明，IMI 偶联 AgNO 具有作为一种强大的抗广泛耐药 抗菌剂的强大潜力。
