Oceanibium sediminis gen. nov., sp. nov., isolated from marine sediment.

A novel Gram-stain-negative, rod-shaped marine bacterium, designated as strain O448, was isolated from the coastal area of Weihai, China (122° 14' E, 36° 54' N). Cells of strain O448 were non-motile, aerobic, approximately 0.4-0.6 µm wide and 1.5-2.0 µm long. Growth occurred at 20-40 °C (optimally between 33-37 °C), pH 6.5-8.0 (optimally at 7.0) and in the presence of 1.0-5.0 % (w/v) NaCl (optimally between 2.0-3.0 %). Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain O448 belongs to family Rhodobacteraceae and exhibits 16S rRNA gene sequence similarities of 94.3, 94.1 and 93.8 % to the type strains of Pontivivens insulae, Halovulum dunhuangense and Maritimibacter alkaliphilus, respectively. The major cellular fatty acid was C18 : 1ω7c and the major polar lipids were phosphatidylethanolamine, phosphatidylcholine and phosphatidylglycerol. Strain O448 contained Q-10 as the sole respiratory quinone. The genomic DNA G+C content was 65.6 mol%. It is evident from phenotypic data and phylogenetic inference that strain O448 represents a novel species in a new genus, for which the name Oceanibiumsediminis gen. nov., sp. nov. is proposed. The type strain is O448 (=KCTC 62076=MCCC 1H00233).