Jung Ji Young, Kang Hye Kyeong, Jin Hyun Mi, Han Sang-Soo, Kwon Young Chul, Eun Jong Jun, Kim Sang Cheol, Seo Min Jeong, Ryu Byung-Gon, Chung Eu Jin
Nakdonggang National Institute of Biological Resources, 137, Donam 2-gil, Sangju-si, Gyeongsangbuk-do 37242, Republic of Korea.
Nonghyup TMR agricultural Co., Ltd., 65, Ogwang 1-gil, Gongseong-myeon, Sangju-si, Gyeongsangbuk-do 37268, Republic of Korea.
Int J Syst Evol Microbiol. 2019 Jun;71(3). doi: 10.1099/ijsem.0.004670. Epub 2021 Jan 27.
A Gram-positive, facultative anaerobic, catalase-negative, non-motile, non-spore-forming and rod-shaped lactic acid bacterium strain, denoted as NFFJ11 and isolated from total mixed fermentation feed in the Republic of Korea, was characterized through polyphasic approaches, including sequence analyses of the 16S rRNA gene and housekeeping genes ( and ), determination of average nucleotide identity and DNA-DNA hybridization, fatty acid methyl ester analysis, and phenotypic characterization. Phylogenetic analyses based on 16S rRNA, and gene sequences revealed that strain NFFJ11 belonged to the genus . The 16S rRNA gene sequence of strain NFFJ11 exhibited high similarity to S215 (99.66 %), Rv4 na (99.53 %), LMG 23699 (99.19 %), YM 0097 (99.06 %), HBUAS52074 (98.86 %) and S4-3 (98.66 %). However, average nucleotide identity and DNA-DNA hybridization values for these type strains were in the range of 79.90-92.93 % and 23.80-49.30 %, respectively, which offer evidence that strain NFFJ11 belongs to a novel species of the genus . The cell-wall peptidoglycan type was A4α (l-Lys-d-Asp) and the G+C content of the genomic DNA was 35.7 mol%. The main fatty acids of strain NFFJ11 were C 9 (43.3 %), C (20.1 %) and summed feature 7 (18.3 %; comprising any combination of C 7, C 6 and C cyclo 10). Through polyphasic taxonomic analysis, it was observed that strain NFFJ11 represents a novel species belonging to the genus , for which the name sp. nov. is proposed. The type strain is NFFJ11 (= KACC 21771= JCM 34088).
从韩国全混合发酵饲料中分离出的一株革兰氏阳性、兼性厌氧、过氧化氢酶阴性、无运动性、不形成芽孢的杆状乳酸菌菌株，命名为NFFJ11，通过多相分类方法进行了鉴定，包括16S rRNA基因和管家基因（和）的序列分析、平均核苷酸同一性测定和DNA-DNA杂交、脂肪酸甲酯分析以及表型特征鉴定。基于16S rRNA、和基因序列的系统发育分析表明，菌株NFFJ11属于属。菌株NFFJ11的16S rRNA基因序列与S215（99.66%）、Rv4 na（99.53%）、LMG 23699（99.19%）、YM 0097（99.06%）、HBUAS52074（98.86%）和S4-3（98.66%）具有高度相似性。然而，这些模式菌株的平均核苷酸同一性和DNA-DNA杂交值分别在79.90 - 92.93%和23.80 - 49.30%范围内，这表明菌株NFFJ11属于属的一个新物种。细胞壁肽聚糖类型为A4α（l-Lys-d-Asp），基因组DNA的G+C含量为35.7 mol%。菌株NFFJ11的主要脂肪酸为C 9（43.3%）、C（20.1%）和总和特征7（18.3%；包括C 7、C 6和C环10的任何组合）。通过多相分类分析，发现菌株NFFJ11代表属的一个新物种，为此提出新名称sp. nov.。模式菌株为NFFJ11（=KACC 21771 =JCM 34088）。
