College of Life Sciences, Northeast Agricultural University, Harbin 150030, PR China.
Int J Syst Evol Microbiol. 2020 Apr;70(4):2467-2484. doi: 10.1099/ijsem.0.004060. Epub 2020 Feb 26.
Fourteen Gram-stain-positive bacterial strains were isolated from Chinese traditional pickle and yogurt. The strains were characterized using a polyphasic taxonomic approach, including 16S rRNA gene sequence analysis, gene sequence analysis, gene sequence analysis, fatty acid methyl ester analysis, determination of DNA G+C content, determination of average nucleotide identity (ANI), DNA-DNA hybridization (DDH) and an analysis of phenotypic features. The data demonstrated that the 14 strains represented ten novel species belonging to the genus , strains 73-4, 247-3, 143-4(a), 33-1, 143-6, 247-4, 17-4, 143-1, 735-2 and M1530-1 were designated as the type strains. Strains 73-4 and 247-3 were phylogenetically related to the type strains of and , having 97.0-98.9 % 16S rRNA gene sequence similarities, 83.9-87.2 % gene sequence similarities and 86.8-93.3 % gene sequence similarities. Strains 143-4(a) and 33-1 were phylogenetically related to the type strains of , and , having 93.6-96.5 % 16S rRNA gene sequence similarities, 73.9-77.2 % gene sequence similarities and 76.1-77.6 % gene sequence similarities. Strains 143-6, 247-4, 17-4 and 143-1 were phylogenetically related to the type strains of , and , exhibiting 95.5-99.9 % 16S rRNA gene sequence similarities, 76.5-83.1 % gene sequence similarities and 83.6-98.3 % gene sequence similarities. Strain 735-2 was phylogenetically related to the type strains of , and , having 98.2-99.1 % 16S rRNA gene sequence similarities, 82.8-84.1 % gene sequence similarities and 93.0-93.9 % gene sequence similarities. Strain M1530-1 was phylogenetically related to the type strains of and , having 99.5 and 99.0 % 16S rRNA gene sequence similarities, 90.3 and 81.7 % gene sequence similarities and 97.7 and 91.1 % gene sequence similarities. The ANI and DDH values between strains 73-4, 247-3, 143-4(a), 33-1, 143-6, 247-4, 17-4, 143-1, 735-2, M1530-1 and type strains of phylogenetically related species were less than 86.8 % and 33.9 % respectively, confirming that they represent ten novel species within the genus . Based upon the data of polyphasic characterization obtained in the present study, ten novel species, sp. nov., sp. nov., sp. nov., sp. nov., sp. nov., sp. nov., sp. nov., sp. nov., sp. nov. and sp. nov., are proposed and the type strains are 73-4 (=NCIMB 15177=CCM 8912=CCTCC AB 2018407), 247-3 (=NCIMB 15176=JCM 33275), 143-4(a) (=NCIMB 15173=CCM 8948=JCM 33273=CCTCC AB 2018390), 33-1 (=NCIMB 15169=CCM 8947=JCM 33272=CCTCC AB 2018405), 143-6 (=NCIMB 15162=CCM 8951=JCM 33274=CCTCC AB 2018411), 247-4 (=NCIMB 15155=CCM 8897=LMG 31059=CCTCC AB 2018410), 17-4 (=NCIMB 15161=CCM 8946=JCM 33271=CCTCC AB 2018406), 143-1 (=NCIMB 15157=CCM 8937=CCTCC AB 2018409), 735-2 (=NCIMB 15190=CCM 8925=LMG 31186) and M1530-1 (=NCIMB 15150=CCM 8893=LMG 31046=CCTCC AB 2018402), respectively.
从中国传统泡菜和酸奶中分离出 14 株革兰氏阳性细菌。这些菌株通过多相分类学方法进行了鉴定，包括 16S rRNA 基因序列分析、基因序列分析、基因序列分析、脂肪酸甲酯分析、DNA G+C 含量测定、平均核苷酸同一性 (ANI) 测定、DNA-DNA 杂交 (DDH) 分析和表型特征分析。数据表明，这 14 株菌代表了 10 个新种，属于 属，菌株 73-4、247-3、143-4(a)、33-1、143-6、247-4、17-4、143-1、735-2 和 M1530-1 被指定为模式菌株。菌株 73-4 和 247-3 与 属的模式菌株亲缘关系较近，其 16S rRNA 基因序列相似性为 97.0-98.9%，基因序列相似性为 83.9-87.2%，基因序列相似性为 86.8-93.3%。菌株 143-4(a)和 33-1 与 属、属和属的模式菌株亲缘关系较近，其 16S rRNA 基因序列相似性为 93.6-96.5%，基因序列相似性为 73.9-77.2%，基因序列相似性为 76.1-77.6%。菌株 143-6、247-4、17-4 和 143-1 与 属、属和属的模式菌株亲缘关系较近，其 16S rRNA 基因序列相似性为 95.5-99.9%，基因序列相似性为 76.5-83.1%，基因序列相似性为 83.6-98.3%。菌株 735-2 与 属、属和属的模式菌株亲缘关系较近，其 16S rRNA 基因序列相似性为 98.2-99.1%，基因序列相似性为 82.8-84.1%，基因序列相似性为 93.0-93.9%。菌株 M1530-1 与 属和属的模式菌株亲缘关系较近，其 16S rRNA 基因序列相似性为 99.5%和 99.0%，基因序列相似性为 90.3%和 81.7%，基因序列相似性为 97.7%和 91.1%。菌株 73-4、247-3、143-4(a)、33-1、143-6、247-4、17-4、143-1、735-2 和 M1530-1 与系统发育上相关的种的模式菌株的 ANI 和 DDH 值均小于 86.8%和 33.9%，证实它们代表了 属内的 10 个新种。基于本研究多相分类特征数据，提出了 10 个新种，分别为 sp. nov.、 sp. nov.、 sp. nov.、 sp. nov.、 sp. nov.、 sp. nov.、 sp. nov.、 sp. nov.、 sp. nov.和 sp. nov.，其模式菌株分别为 73-4(=NCIMB 15177=CCM 8912=CCTCC AB 2018407)、247-3(=NCIMB 15176=JCM 33275)、143-4(a)(=NCIMB 15173=CCM 8948=JCM 33273=CCTCC AB 2018390)、33-1(=NCIMB 15169=CCM 8947=JCM 33272=CCTCC AB 2018405)、143-6(=NCIMB 15162=CCM 8951=JCM 33274=CCTCC AB 2018411)、247-4(=NCIMB 15155=CCM 8897=LMG 31059=CCTCC AB 2018410)、17-4(=NCIMB 15161=CCM 8946=JCM 33271=CCTCC AB 2018406)、143-1(=NCIMB 15157=CCM 8937=CCTCC AB 2018409)、735-2(=NCIMB 15190=CCM 8925=LMG 31186)和 M1530-1(=NCIMB 15150=CCM 8893=LMG 31046=CCTCC AB 2018402)。
