School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
Anal Chem. 2021 Feb 9;93(5):3042-3051. doi: 10.1021/acs.analchem.0c05200. Epub 2021 Jan 27.
Chemiluminescence (CL) resonance energy transfer (CRET) has received great attention due to its fascinating applications in imaging and photodynamic therapy. Here, we report a highly efficient CRET polymer dot ()-based system using catalytic CL reagents as energy donors and fluorescent polymers and dyes as energy acceptors. consist of Fe(III) deuteroporphyrin IX (CL catalyst), fluorescent polymers, and dyes. The CL intensity and duration are markedly enhanced by using ultrasensitive catalytic CL reaction of luminol analogue-HO, and the CL emission wavelength can be adjusted by one-step/two-step energy-transfer strategies. show intensive multicolor CL (about 3000× enhanced), an adjustable emission wavelength (470-720 nm), long CL duration (over 8 h), and a high CRET efficiency (50%). possess excellent biocompatibility, sensitive response to reactive oxygen species (ROS), and ultrahigh catalytic activity. They are successfully used for high-contrast real-time ROS imaging and tumor-targeted imaging with an excellent signal-to-noise ratio (over 90).
化学发光（CL）共振能量转移（CRET）由于其在成像和光动力治疗中的迷人应用而受到极大关注。在这里，我们报告了一种基于高效 CRET 聚合物点（）的系统，该系统使用催化 CL 试剂作为能量供体，荧光聚合物和染料作为能量受体。聚合物点由 Fe(III) 原卟啉 IX（CL 催化剂）、荧光聚合物和染料组成。通过使用超灵敏的发光体类似物-HO 的催化 CL 反应，显著增强了 CL 强度和持续时间，并且可以通过一步/两步能量转移策略来调节 CL 发射波长。聚合物点表现出强烈的多色 CL（增强约 3000 倍）、可调发射波长（470-720nm）、长 CL 持续时间（超过 8 小时）和高 CRET 效率（50%）。聚合物点具有良好的生物相容性、对活性氧（ROS）的敏感响应和超高的催化活性。它们成功地用于高对比度实时 ROS 成像和具有出色信噪比（超过 90）的肿瘤靶向成像。
