Snak Aline, Vendruscolo Eliane Cristina Gruszka, Santos Marise Fonseca Dos, Fiorini Adriana, Mesa Dany
Universidade Federal do Paraná, Labiogen-Laboratório de Bioquímica e Genética, Palotina, PR, Brazil.
Universidade Federal do Paraná, Departamento de Biociências, Palotina, PR, Brazil.
Genet Mol Biol. 2021 Jan 27;44(1):e20200130. doi: 10.1590/1678-4685-GMB-2020-0130. eCollection 2021.
Plant growth-promoting bacteria are ecological alternatives for fertilization, mainly for gramineous. Since plant x bacteria interaction is genotype and strain dependent, searching for new strains may contribute to the development of new biofertilizers. We aim to characterize plant growth-promoting capacity of Leclercia adecarboxylata strain Palotina, formerly isolated by our group in corn. A single isolated colony was taken and its genome was sequenced using Illumina technology. The whole genome was compared to other Leclercia adecarboxylata strains, and their biological and growth-promoting traits, such as P solubilization and auxin production, were tested. Following that, a 4.8 Mb genome of L. adecarboxylata strain Palotina was assembled and the functional annotation was carried out. This paper is the first to report the genes associated with plant growth promotion demonstrating in vitro indole acid production by this strain. These results project the endophyte as a potential biofertilizer for further commercial exploitation.
促进植物生长的细菌是施肥的生态替代方案，主要适用于禾本科植物。由于植物与细菌的相互作用取决于基因型和菌株，寻找新菌株可能有助于新型生物肥料的开发。我们旨在表征先前由我们团队从玉米中分离出的脱羧勒克氏菌菌株帕洛蒂纳促进植物生长的能力。选取了一个单菌落，并用Illumina技术对其基因组进行测序。将整个基因组与其他脱羧勒克氏菌菌株进行比较，并测试它们的生物学和促进生长特性，如磷溶解和生长素产生。在此之后，组装了脱羧勒克氏菌菌株帕洛蒂纳的4.8 Mb基因组并进行了功能注释。本文首次报道了与植物生长促进相关的基因，证明了该菌株在体外产生吲哚酸。这些结果表明该内生菌是一种有潜力的生物肥料，可用于进一步的商业开发。
