Viteri Jumbo Luis O, Moura Wellington S, Possel Richard D, Herrera Osmany M, Fidelis Rodrigo R, Andrade Bruno S, Smagghe Guy, Santos Gil R, Oliveira Eugênio E, Aguiar Raimundo W S
Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Tocantins (UFT), Gurupi 77402-970, TO, Brazil.
Programa de Pós-Graduação em Produção Vegetal, Universidade Federal de Tocantins (UFT), Gurupi 77402-970, TO, Brazil.
Toxins (Basel). 2025 Aug 11;17(8):402. doi: 10.3390/toxins17080402.
, also known as the yellow fever mosquito, presents a major public health challenge, highlighting the need for effective biorational agents for mosquito control. Here, we investigated the synergistic effects of essential oil mixtures derived from that is a mint-family shrub native to Brazil's Cerrado biome, known as "alecrim do Cerrado", in combination with essential oils from noni (), Brazilian mint ("salva-do-Marajó", ), and lemongrass () against . We conducted phytochemical analyses and assessed larvicidal, repellent, and oviposition deterrent activities. Using in silico methods, we predicted molecular interactions between key essential oil components and physiological targets involved in repellent action (odorant-binding protein AeagOBP1 and olfactory receptor Or31) and larvicidal activity (GABA and octopamine receptors, TRP channels, and acetylcholinesterase [AChE]). Major compounds identified included octanoic acid (23%; × ), 2,5-dimethoxy--cymene (21.9%; × ), and citral (23.0%; × ). Although individual oils showed strong larvicidal activity ( LC = 2.35 µL/mL; = 2.37 µL/mL; and = 2.71 µL/mL), their mixtures did not display synergistic effects. Similarly, repellency and oviposition deterrence were comparable to DEET for individual oils but were not enhanced in mixtures. Notably, the × essential oil blend reduced oviposition deterrence. Molecular docking confirmed strong binding of major oil components to AeagOBP1 and Or31, supporting their role in repellency. For larvicidal effects, AChE showed the highest predicted binding affinity. Overall, our findings suggest that , , , and (alone or in 1:1 mixture) are promising, sustainable agents for control.
，也被称为黄热病蚊子，带来了重大的公共卫生挑战，凸显了对有效生物合理制剂用于蚊虫控制的需求。在此，我们研究了源自一种原产于巴西塞拉多生物群落的薄荷科灌木（被称为“塞拉多百里香”）的精油混合物，与诺丽（）、巴西薄荷（“马拉若岛鼠尾草”，）和柠檬草（）的精油联合对的协同作用。我们进行了植物化学分析，并评估了杀幼虫、驱避和产卵抑制活性。使用计算机模拟方法，我们预测了关键精油成分与驱避作用（气味结合蛋白AeagOBP1和嗅觉受体Or31）和杀幼虫活性（GABA和章鱼胺受体、TRP通道以及乙酰胆碱酯酶[AChE]）所涉及的生理靶点之间的分子相互作用。鉴定出的主要化合物包括辛酸（23%；×）、2,5 - 二甲氧基 - 对异丙基苯（21.9%；×）和柠檬醛（23.0%；×）。尽管各单一精油显示出较强的杀幼虫活性（淡色库蚊LC₅₀ = 2.35 μL/mL；埃及伊蚊 = 2.37 μL/mL；白纹伊蚊和致倦库蚊 = 2.71 μL/mL），但其混合物并未显示出协同效应。同样，各单一精油的驱避和产卵抑制效果与避蚊胺相当，但混合物中并未增强。值得注意的是，×精油混合物降低了产卵抑制作用。分子对接证实了主要精油成分与AeagOBP1和Or31的强结合，支持了它们在驱避中的作用。对于杀幼虫作用，AChE显示出最高的预测结合亲和力。总体而言，我们的研究结果表明，、、和（单独或1:1混合）是用于蚊虫控制的有前景的可持续制剂。
