Lima Glauber Cruz, Vasconcelos Yuri de Abreu Gomes, de Santana Souza Marilia Trindade, Oliveira Alan Santos, Bomfim Rangel Rodrigues, de Albuquerque Júnior Ricardo Luiz Cavalcanti, Camargo Enilton Aparecido, Portella Viviane Gomes, Coelho-de-Souza Andrelina Noronha, Diniz Lúcio Ricardo Leite
1 Department of Physiology, Superior Institute of Biomedical Sciences, State University of Ceará , Fortaleza, Ceará, Brazil .
2 Department of Physiology, Federal University of Sergipe , São Cristóvão, Sergipe, Brazil .
J Med Food. 2018 Jul;21(7):709-715. doi: 10.1089/jmf.2017.0125. Epub 2018 Feb 28.
No specific therapeutics are available for the treatment of sepsis-induced liver dysfunction, a clinical complication strongly associated with the high mortality rate of septic patients. This study investigated the effect of the essential oil of Hyptis crenata (EOHc), a lamiaceae plant used to treat liver disturbances in Brazilian folk medicine, on liver function during early sepsis. Sepsis was induced by the cecal ligation and puncture (CLP) model. Rats were divided into four groups: Sham, Sham+EOHc, CLP, and CLP+EOHc. EOHc (300 mg/kg) was orally administered 12 and 24 h after surgery. The animals were sacrificed for blood collection and liver tissue samples 48 h after surgery. Hepatic function was evaluated by measuring serum bilirubin, alkaline phosphatase (ALP), aspartate aminotransferase, and alanine aminotransferase (ALT) levels. The levels of malondialdehyde and the activity of superoxide dismutase, catalase, and GSH peroxidase (GSH-Px) were measured for assessment of oxidative stress. Liver morphology was analyzed by hematoxylin and eosin staining. EOHc normalized serum ALP, ALT, and bilirubin levels and inhibited morphological changes. In addition, we observed that EOHc inhibited elevation in hepatic lipid peroxidation and reduction of the glutathione peroxidase activity induced by sepsis. Our data show that EOHc plays a protective effect against liver injury induced by sepsis.
目前尚无特异性疗法可用于治疗脓毒症诱导的肝功能障碍，这是一种与脓毒症患者高死亡率密切相关的临床并发症。本研究调查了唇形科植物皱叶香科科（Hyptis crenata）精油（EOHc）对早期脓毒症期间肝功能的影响，该植物在巴西民间医学中用于治疗肝脏疾病。采用盲肠结扎穿刺（CLP）模型诱导脓毒症。将大鼠分为四组：假手术组、假手术+EOHc组、CLP组和CLP+EOHc组。术后12小时和24小时口服EOHc（300mg/kg）。术后48小时处死动物以采集血液和肝脏组织样本。通过测量血清胆红素、碱性磷酸酶（ALP）、天冬氨酸转氨酶和丙氨酸转氨酶（ALT）水平评估肝功能。测量丙二醛水平以及超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶（GSH-Px）的活性以评估氧化应激。通过苏木精和伊红染色分析肝脏形态。EOHc使血清ALP、ALT和胆红素水平恢复正常，并抑制形态学变化。此外，我们观察到EOHc抑制了脓毒症诱导的肝脏脂质过氧化升高和谷胱甘肽过氧化物酶活性降低。我们的数据表明，EOHc对脓毒症诱导的肝损伤具有保护作用。
