Fatahi Arghavan, Soleimani Neda, Afrough Parviz
Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.
Department of Mycobacteriology & Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.
Int J Food Sci. 2021 Jan 12;2021:8180742. doi: 10.1155/2021/8180742. eCollection 2021.
Kefir drink is one of the most important probiotic products, which is made using kefir microorganisms in fermenting the milk. Numerous investigation have been accomplished in the field of the therapeutic property of probiotic products. In the present study, we assessed the cytotoxic effect of kefir on the rate of growth and increase of glioblastoma cancer cell as the most severe form of brain tumors. In this experimental study, we used a U87 cancer cell line (glioblastoma). The interaction between cancer cells and different concentrations of kefir drink and supernatants at 24 and 48 hours was considered. The cell cytotoxicity of kefir and sedimentation of cell lysate and extract of kefir was assessed using the MTT test after 24 and 48 hours. The result of the MTT test, treatment of the cells with the 48-hour fermented drink, demonstrated the most cell cytotoxicity in comparison with the control group. Results showed that the toxicity effect in all groups was dose-dependent, and by increasing the concentration, cell survival decreased noticeably. The results indicated that the supernatant of fermented kefir drink as a probiotic product has more toxicity and lethality effect on the glioblastoma cancer cell. This product can be utilized as a replacement or a complementary therapy of cancer.
开菲尔饮品是最重要的益生菌产品之一，它是利用开菲尔微生物发酵牛奶制成的。在益生菌产品治疗特性领域已经完成了大量研究。在本研究中，我们评估了开菲尔对胶质母细胞瘤癌细胞（最严重的脑肿瘤形式）生长速率和增殖的细胞毒性作用。在这项实验研究中，我们使用了U87癌细胞系（胶质母细胞瘤）。研究了癌细胞与不同浓度的开菲尔饮品及其上清液在24小时和48小时时的相互作用。在24小时和48小时后，使用MTT试验评估开菲尔的细胞毒性以及细胞裂解物和开菲尔提取物的沉淀情况。MTT试验结果表明，与对照组相比，用48小时发酵饮品处理细胞时细胞毒性最大。结果显示，所有组的毒性作用均呈剂量依赖性，随着浓度增加，细胞存活率显著降低。结果表明，作为益生菌产品的发酵开菲尔饮品上清液对胶质母细胞瘤癌细胞具有更大的毒性和致死作用。该产品可作为癌症的替代或辅助治疗手段。
