Holmes B, Costas M, Sloss L L
National Collection of Type Cultures, Central Public Health Laboratory, London, UK.
J Appl Bacteriol. 1988 Jan;64(1):27-35. doi: 10.1111/j.1365-2672.1988.tb02426.x.
Twenty-five strains of Providencia alcalifaciens from various countries have been characterized by one-dimensional SDS-PAGE of cellular proteins. They comprised 15 from human faeces, one from duck faeces, one from a guinea-pig eye and eight from unknown sources. Also included, for reference purposes, were the type strains of three other Providencia species. The protein patterns, which contained 45-50 discrete bands, were highly reproducible and were used as the basis for two numerical analyses. In the first, in which the principal protein bands (in the 33-40 kD range) were excluded, the 25 Prov. alcalifaciens strains formed, at the 83% S level, a single cluster whilst the three Providencia reference strains remained unclustered. In the second, which included all the protein bands, the 25 Prov. alcalifaciens strains formed 10 clusters at the 85% S level. We conclude that high resolution PAGE combined with computerized analysis of protein patterns provides the basis for typing clinical strains of Prov. alcalifaciens. Reference strains of each of the 10 PAGE types identified are available from NCTC for inclusion in future studies.
已通过细胞蛋白的一维SDS - PAGE对来自不同国家的25株产碱普罗威登斯菌进行了特征分析。其中15株来自人类粪便，1株来自鸭粪便，1株来自豚鼠眼睛，8株来源不明。另外，为作参考还纳入了其他三种普罗威登斯菌的模式菌株。蛋白质图谱包含45 - 50条离散条带，具有高度可重复性，并作为两种数值分析的基础。在第一种分析中，排除了主要蛋白条带（33 - 40 kD范围内），25株产碱普罗威登斯菌菌株在相似度83%的水平上形成了一个单一聚类，而三种普罗威登斯菌参考菌株仍未聚类。在第二种分析中，纳入了所有蛋白条带，25株产碱普罗威登斯菌菌株在相似度85%的水平上形成了10个聚类。我们得出结论，高分辨率PAGE结合蛋白质图谱的计算机分析为产碱普罗威登斯菌临床菌株的分型提供了基础。已鉴定的10种PAGE类型中的每种参考菌株均可从英国国家典型培养物保藏中心获得，以供未来研究使用。
