Bogusz M, Aderjan R
Institute of Forensic Medicine, Karl-Ruprecht-University, Heidelberg, F.R.G.
J Chromatogr. 1988 Jan 1;435(1):43-53. doi: 10.1016/s0021-9673(01)82161-0.
A retention index scale based on 1-nitroalkanes was introduced for reversed-phase high-performance liquid chromatography. The retention behaviour of nitroalkanes was examined using two different ODS-silica columns. The nitroalkanes are stable compounds having high UV absorbances at 200-220 nm. The correlation between the logarithm of the capacity factor and the carbon number was linear from the second homologue. The compounds up to 1-nitrohexane are easily available commercially, and they cover the retention range for the majority of toxicologically relevant substances. The retention indices of several test compounds showed that the scale based on 1-nitroalkanes is comparable to that based on alkyl aryl ketones, and additionally may cover the early eluted compounds, for which reference compounds on the alkyl aryl ketone scale are not available.
介绍了一种基于1-硝基烷烃的保留指数标度用于反相高效液相色谱法。使用两种不同的ODS-硅胶柱考察了硝基烷烃的保留行为。硝基烷烃是在200 - 220 nm处具有高紫外吸收的稳定化合物。从第二个同系物开始，容量因子的对数与碳原子数之间的相关性呈线性。直至1-硝基己烷的化合物在商业上易于获得，并且它们涵盖了大多数毒理学相关物质的保留范围。几种测试化合物的保留指数表明，基于1-硝基烷烃的标度与基于烷基芳基酮的标度相当，并且此外还可能涵盖早期洗脱的化合物，对于这些化合物，烷基芳基酮标度上没有参考化合物。
