Reversed-phase high-performance liquid chromatographic database of retention indices and UV spectra of toxicologically relevant substances and its interlaboratory use.

An HPLC identification system based on a 1-nitroalkane retention index scale and secondary retention index standards is described. The retention index values and spectral data for 383 toxicologically relevant compounds (therapeutic and illicit drugs, environmental toxins and endogenous compounds) are given. The retention data may be directly applied in any laboratory using any reversed-phase, base-deactivated column, provided that the standardized procedure is observed.