Davies E T, Nasaruddin B A, Alhaq A, Senaldi G, Vergani D
Department of Immunology, King's College School of Medicine and Dentistry, London.
J Clin Pathol. 1988 Feb;41(2):143-7. doi: 10.1136/jcp.41.2.143.
A new laser nephelometric technique that measures C4d for the assessment of the activation of the classical complement pathway was developed. C4d was isolated from other larger C4 related molecules at a final concentration of polyethylene glycol of 12% and then quantitated by laser nephelometry using a commercially available antiserum, which reacts with C4d determinants. C4d standard (100%) was produced by exhaustive activation of the classical pathway in pooled normal human serum using heat aggregated human immunoglobulin. Serial dilutions of the standard provided a reference curve against which clinical samples were read. Patients with rheumatoid arthritis showed significantly higher C4d values (mean 53.8%) than controls (21.7%; p less than 0.001). The technique proved accurate, rapid, and suitable for the routine laboratory evaluation of complement activation through the classical pathway, and it may be useful in the management of those conditions in which complement activation has a pathogenic role.
开发了一种新的激光散射比浊技术，用于测量C4d以评估经典补体途径的激活情况。在聚乙二醇终浓度为12%的条件下，从其他较大的C4相关分子中分离出C4d，然后使用与C4d决定簇反应的市售抗血清通过激光散射比浊法定量。通过使用热聚集人免疫球蛋白彻底激活混合正常人血清中的经典途径来制备C4d标准品（100%）。标准品的系列稀释提供了一条参考曲线，据此读取临床样本。类风湿性关节炎患者的C4d值（平均53.8%）显著高于对照组（21.7%；p<0.001）。该技术被证明准确、快速，适用于通过经典途径进行补体激活的常规实验室评估，并且可能有助于管理那些补体激活具有致病作用的疾病。
