LEO Pharma A/S, Ballerup, Denmark.
FibroTx, Tallinn, Estonia.
Skin Res Technol. 2021 Sep;27(5):715-722. doi: 10.1111/srt.13006. Epub 2021 Jan 28.
Skin biomarkers are important tools for characterizing specific disease processes in atopic dermatitis (AD) patients and can be used for monitoring and potentially predicting treatment response. Recent developments of minimally invasive skin sampling methods have made sampling easier and less inconvenient for patients. The objective of this study was to evaluate the non-invasive patch technique developed by FibroTx for skin biomarker analysis.
Ten adult patients with AD were included in the study and treated with topical corticosteroid (diprosone 0.05%) for 2 weeks. Skin surface biomarkers were assessed in three lesional and non-lesional sites before and during treatment using the FibroTx Patch method. Skin tape strips were also collected from the subjects for comparison.
The results showed expression of IL-1 cytokine family members, chemokines, and defensins on lesional and non-lesional skin. Several of these markers were strongly reduced by topical treatment. The biomarker expression in skin surface eluates correlated strongly with those seen in skin tape strips from the same subjects.
These data further support the usefulness of non-invasive sampling methods for assessing inflammatory processes in AD skin and demonstrate that the patch sampling method is a good alternative to skin tape strips.
皮肤生物标志物是表征特应性皮炎（AD）患者特定疾病过程的重要工具，可用于监测和潜在预测治疗反应。最近微创皮肤采样方法的发展使得患者的采样更加容易和方便。本研究的目的是评估 FibroTx 开发的用于皮肤生物标志物分析的非侵入性贴剂技术。
研究纳入了 10 名成人 AD 患者，并在 2 周内接受外用皮质类固醇（地奈德 0.05%）治疗。使用 FibroTx 贴剂法在治疗前和治疗期间评估三个病变和非病变部位的皮肤表面生物标志物。还收集了受试者的皮肤胶带条进行比较。
结果显示在病变和非病变皮肤上表达了 IL-1 细胞因子家族成员、趋化因子和防御素。这些标志物中的几种经局部治疗后明显减少。皮肤表面洗脱物中的生物标志物表达与来自同一受试者的皮肤胶带条上观察到的表达密切相关。
这些数据进一步支持了非侵入性采样方法在评估 AD 皮肤炎症过程中的有用性，并表明贴剂采样方法是皮肤胶带条的良好替代品。
