Université de Monastir, Institut Supérieur de Biotechnologie de Monastir, Laboratoire LR11ES41 Génétique Biodiversité et Valorisation des Bio-Ressources, 5000, Monastir, Tunisie.
Spine Deform. 2021 Jul;9(4):883-892. doi: 10.1007/s43390-021-00291-w. Epub 2021 Jan 29.
Observational study of Killifish with spinal deformities OBJECTIVE: To evaluate the morphology and molecular biology of Aphanius fasciatus with severe spine deformities. Idiopathic Scoliosis affects 3% of the population and is an abnormal three-dimensional curvature of the spine with unknown cause. The lack of a model system with naturally occurring spinal curvatures has hindered research on the etiology of IS.
The Mediterranean killifish Aphanius fasciatus, collected from the coast of Sfax (Tunisia), which has an inborn skeletal deformity was chosen. We used morphologic features to evaluate the severity of scoliosis according to the different types and performed a biochemical analysis using factors previously studied in humans (estradiol, melatonin and Insulin Growth Factor 1 "IGF-1").
We have detected relevant molecular deviations that occur in Killifish deformities and the fish with severe scoliosis are smaller and less old than the ones with milder scolioses. Furthermore, a significant change in levels of ovarian estradiol, liver IGF-1 and brain melatonin was noted between deformed and normal fish.
Aphanius fasciatus could be used as a molecular model system to study the etiology of IS in humans as the characterization of the Aphanius fasciatus scoliosis syndrome has revealed morphological and biochemical parallels to IS. However, it is important to note the limitations of the proposed model, including the short lifespan of the fish.
III.
观察具有脊柱畸形的食蚊鱼
评估严重脊柱畸形的 Aphanius fasciatus 的形态和分子生物学。特发性脊柱侧凸影响 3%的人口，是一种未知原因的脊柱异常三维弯曲。缺乏具有自然发生脊柱弯曲的模型系统，阻碍了对 IS 病因的研究。
选择来自突尼斯斯法克斯海岸的地中海食蚊鱼 Aphanius fasciatus，它具有先天的骨骼畸形。我们根据不同类型使用形态特征来评估脊柱侧凸的严重程度，并使用先前在人类中研究过的因素（雌二醇、褪黑素和胰岛素样生长因子 1“IGF-1”）进行生化分析。
我们已经检测到食蚊鱼畸形中发生的相关分子偏差，并且严重脊柱侧凸的鱼比轻度脊柱侧凸的鱼更小且更年轻。此外，还观察到畸形鱼和正常鱼之间卵巢雌二醇、肝脏 IGF-1 和大脑褪黑素水平有显著变化。
Aphanius fasciatus 可作为研究人类 IS 病因的分子模型系统，因为 Aphanius fasciatus 脊柱侧凸综合征的特征与 IS 具有形态和生化相似性。然而，需要注意所提出模型的局限性，包括鱼的寿命较短。
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