Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology, 56 Lingyuanxi Road, Guangzhou 510055, China.
Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology, 56 Lingyuanxi Road, Guangzhou 510055, China.
Life Sci. 2021 Apr 1;270:119125. doi: 10.1016/j.lfs.2021.119125. Epub 2021 Jan 26.
Human periodontal ligament stem cells (hPDLSCs) tether the teeth to the surrounding bone and are considered as major functional stem cells responsible for regeneration of the alveolar bone and periodontal ligament tissue. However, the outcome of stem cell regenerative therapy is affected by the survival rate and their differentiation potential of transplanted cells. This is primarily because of local oxidative stress and chronic inflammation at the transplantation site. Therefore, our study aimed to explore whether a natural antioxidant, curcumin could increase the tissue regeneration ability of transplanted hPDLSCs.
A hydrogen peroxide environment and a rat cranial bone defect model were built to mimic the oxidative stress conditions in vitro and in vivo, respectively. We evaluated the effect of curcumin on oxidative status, apoptosis, mitochondrial function and osteogenic differentiation of HO-stimulated hPDLSCs in vitro. We also measured the effect of curcumin on cell viability and bone repair ability of transplanted hPDLSCs in vivo.
Our data showed that curcumin enhanced cell proliferation, reduced the reactive oxygen species (ROS) levels and apoptosis, maintained the standard mitochondrial structure and function, and promoted osteogenic differentiation of HO-stimulated hPDLSCs. The extracellular regulated protein kinases 1/2 (Erk1/2) signaling pathway was determined to be involved in the osteogenic differentiation of the HO-stimulated hPDLSCs. Moreover, curcumin enhanced the viability and the bone repair ability of hPDLSCs in vivo.
Curcumin reduced apoptosis and promoted osteogenesis of the hPDLSCs under oxidative stress, and might therefore have a potential clinical use with respect to tissue regeneration.
人牙周韧带干细胞（hPDLSCs）将牙齿固定在周围的骨骼上，被认为是主要的功能性干细胞，负责再生牙槽骨和牙周韧带组织。然而，干细胞再生治疗的效果受到移植细胞的存活率和分化潜能的影响。这主要是由于移植部位的局部氧化应激和慢性炎症。因此，我们的研究旨在探讨天然抗氧化剂姜黄素是否能提高移植的 hPDLSCs 的组织再生能力。
建立了过氧化氢环境和大鼠颅骨缺损模型，分别模拟体外和体内的氧化应激条件。我们评估了姜黄素对 HO 刺激的 hPDLSCs 中氧化状态、细胞凋亡、线粒体功能和成骨分化的影响。我们还测量了姜黄素对移植的 hPDLSCs 细胞活力和骨修复能力的影响。
我们的数据表明，姜黄素增强了细胞增殖，降低了活性氧（ROS）水平和细胞凋亡，维持了标准的线粒体结构和功能，并促进了 HO 刺激的 hPDLSCs 的成骨分化。细胞外调节蛋白激酶 1/2（Erk1/2）信号通路被确定参与了 HO 刺激的 hPDLSCs 的成骨分化。此外，姜黄素增强了 hPDLSCs 在体内的活力和骨修复能力。
姜黄素减少了 hPDLSCs 在氧化应激下的细胞凋亡并促进了其成骨作用，因此可能具有组织再生的潜在临床应用价值。
