Department of Veterinary Medicine, University of Bari "Aldo Moro", Str. Prov. per Casamassima Km 3, 70010 Valenzano, BA, Italy.
Department of Veterinary Medicine, University of Bari "Aldo Moro", Str. Prov. per Casamassima Km 3, 70010 Valenzano, BA, Italy.
Mar Pollut Bull. 2021 Mar;164:112015. doi: 10.1016/j.marpolbul.2021.112015. Epub 2021 Jan 26.
Sea turtles are useful sentinels to monitor the dissemination of antimicrobial resistance (AMR) in the marine coastal ecosystems. Forty Gram negative bacteria were isolated from wounds of 52 injured Caretta caretta, living in the Mediterranean Sea. Bacteria were identified using 16S rRNA gene sequencing and tested for susceptibility to 15 antibiotics. In addition, NGS amplicon sequencing was performed to detect the presence of AmpC β-lactamase genes (bla) and extended-spectrum β-lactamase (ESBL) genes (blablabla). Seventy-five percent of the isolates (30/40 isolates) exhibited multidrug resistance (MDR) phenotypes and 32.5% (13/40 isolates) were confirmed to be positive for at least one gene. The variants of ESBLs genes were blabla and bla. Variants of the blaβ-lactamase gene i.e., bla, bla, bla, bla and bla, were also detected. In addition, 4 isolates were found simultaneously harboring CTX and AmpC genes while 2 strains harbored 3 genes (bla, and bla).
海龟是监测海洋沿海生态系统中抗生素耐药性（AMR）传播的有用哨兵。从生活在地中海的 52 只受伤的 Caretta caretta 海龟的伤口中分离出 40 株革兰氏阴性菌。使用 16S rRNA 基因测序鉴定细菌，并测试对 15 种抗生素的敏感性。此外，还进行了 NGS 扩增子测序，以检测 AmpC β-内酰胺酶基因（bla）和超广谱β-内酰胺酶（ESBL）基因（blablabla）的存在。75%（30/40 株）的分离株表现出多药耐药（MDR）表型，32.5%（13/40 株）的分离株至少有一种基因呈阳性。ESBLs 基因的变体为 blabla 和 bla。还检测到 blaβ-内酰胺酶基因的变体，即 bla、bla、bla、bla 和 bla。此外，有 4 株同时携带 CTX 和 AmpC 基因，有 2 株携带 3 种基因（bla、bla 和 bla）。
