Hammoud Alissa, Louni Meriem, Baldé Mamadou Cellou, Beavogui Abdoul Habib, Gautret Philippe, Raoult Didier, Fenollar Florence, Misse Dorothée, Mediannikov Oleg
Aix Marseille University, IRD, APHM, MEPHI, IHU-Méditerranée Infection, 13005 Marseille, France.
Aix Marseille University, IRD, APHM, VITROME, IHU-Méditerranée Infection, 13005 Marseille, France.
Microorganisms. 2021 Jan 27;9(2):257. doi: 10.3390/microorganisms9020257.
, the head louse, is an obligate blood-sucking ectoparasite that occurs in six divergent mitochondrial clades (A, D, B, F, C and E). Several studies reported the presence of different pathogenic agents in head lice specimens collected worldwide. These findings suggest that head louse could be a dangerous vector and a serious public health problem. Herein, we aimed to study the mitochondrial genetic diversity, the PHUM540560 gene polymorphisms profile of head lice collected in Guinea, as well as to screen for their associated pathogens. In 2018, a total of 155 head lice were collected from 49 individuals at the Medicals Centers of rural (Maférinyah village) and urban (Kindia city) areas, in Guinea. Specimens were subjected to a genetic analysis and pathogens screening using molecular tools. Results showed that all head lice belonged to eight haplotypes in the E haplogroup, with six newly identified for the first time. The study of the PHUM540560 gene polymorphisms of our clade E-head lice revealed that 82.5% exhibited the same polymorphism profile as the previously reported clade A-body lice. Screening for targeted pathogens revealed the presence of spp., while sequencing highlighted the presence of several species, including , and for the first time . Our study is the first to report the existence of the Guinean haplogroup E, the PHUM540560 gene polymorphism profile as well as the presence of species in head lice collected from Guinea.
头虱是一种专性吸血体外寄生虫，存在于六个不同的线粒体进化枝（A、D、B、F、C和E）中。多项研究报告称，在全球收集的头虱标本中存在不同的病原体。这些发现表明，头虱可能是一种危险的病媒，也是一个严重的公共卫生问题。在此，我们旨在研究几内亚收集的头虱的线粒体遗传多样性、PHUM540560基因多态性概况，并筛选其相关病原体。2018年，在几内亚农村（马费里尼亚村）和城市（金迪亚市）地区的医疗中心，从49个人身上共收集了155只头虱。使用分子工具对标本进行遗传分析和病原体筛选。结果表明，所有头虱都属于E单倍群中的八个单倍型，其中六个是首次新鉴定出来的。对我们的E进化枝头虱的PHUM540560基因多态性研究表明，82.5%的头虱表现出与先前报道的A进化枝体虱相同的多态性概况。对目标病原体的筛选发现了 spp.的存在，而测序突出了几种物种的存在，包括 ， ，并且首次发现了 。我们的研究首次报告了几内亚单倍群E的存在、PHUM540560基因多态性概况以及从几内亚收集的头虱中 物种的存在。
