Institute of Molecular and Industrial Biotechnology, Lodz University of Technology, B. Stefanowskiego 4/10, 90-924, Lodz, Poland.
Faculty of Process and Environmental Engineering, Department of Bioprocess Engineering, Lodz University of Technology, ul. Wolczanska 213, 90-924, Lodz, Poland.
Appl Microbiol Biotechnol. 2021 Feb;105(4):1615-1627. doi: 10.1007/s00253-021-11133-0. Epub 2021 Jan 30.
In the present study, the complete genome of a filamentous fungus Aspergillus terreus ATCC 20542 was sequenced, assembled, and annotated. This strain is mainly recognized for being a model wild-type lovastatin producer and a parental strain of high-yielding industrial mutants. It is also a microorganism with a rich repertoire of secondary metabolites that has been a subject of numerous bioprocess-related studies. In terms of continuity, the genomic sequence provided in this work is of the highest quality among all the publicly available genomes of A. terreus strains. The comparative analysis revealed considerable diversity with regard to the catalog of biosynthetic gene clusters found in A. terreus. Even though the cluster of lovastatin biosynthesis was found to be well-conserved at the species level, several unique genes putatively associated with metabolic functions were detected in A. terreus ATCC 20542 that were not detected in other investigated genomes. The analysis was conducted also in the context of the primary metabolic pathways (sugar catabolism, biomass degradation potential, organic acid production), where the visible differences in gene copy numbers were detected. However, the species-level genomic diversity of A. terreus was more evident for secondary metabolism than for the well-conserved primary metabolic pathways. The newly sequenced genome of A. terreus ATCC 20542 was found to harbor several unique sequences, which can be regarded as interesting subjects for future experimental efforts on A. terreus metabolism and fungal biosynthetic capabilities. KEY POINTS: • The high-quality genome of Aspergillus terreus ATCC 20542 has been assembled and annotated. • Comparative analysis with other sequenced Aspergillus terreus strains has revealed considerable diversity in biosynthetic gene repertoire, especially related to secondary metabolism. • The unique genomic features of A. terreus ATCC 20542 are discussed.
在本研究中，对丝状真菌土曲霉 ATCC 20542 的全基因组进行了测序、组装和注释。该菌株主要作为洛伐他汀生产的模型野生型和高产工业突变株的亲本菌株而被广泛认可。它还是一种具有丰富次生代谢产物的微生物，已经成为许多与生物过程相关研究的主题。就连续性而言，本工作提供的基因组序列是所有已公开的土曲霉菌株基因组中质量最高的。比较分析显示，土曲霉中生物合成基因簇的目录存在很大的多样性。尽管洛伐他汀生物合成簇在种水平上被发现是保守的，但在 ATCC 20542 中检测到了一些与代谢功能相关的推定独特基因，而在其他研究的基因组中未检测到。还对初级代谢途径（糖分解代谢、生物质降解潜力、有机酸生产）进行了分析，在这些途径中检测到基因拷贝数的明显差异。然而，与保守的初级代谢途径相比，土曲霉的种水平基因组多样性在次生代谢中更为明显。新测序的 ATCC 20542 土曲霉基因组被发现含有几个独特的序列，这些序列可以被视为未来土曲霉代谢和真菌生物合成能力实验研究的有趣对象。关键点：
Aspergillus terreus ATCC 20542 的高质量基因组已组装和注释。
与其他已测序的 Aspergillus terreus 菌株进行比较分析，揭示了生物合成基因库的巨大多样性，特别是与次生代谢相关的多样性。
讨论了 ATCC 20542 土曲霉的独特基因组特征。
