不同酶组合制备多孔淀粉的功能性质比较。
Comparison of functional properties of porous starches produced with different enzyme combinations.
机构信息
State Key Laboratory of Biobased Material and Green Papermaking, School of Food Sciences and Engineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan, China.
Life Science and Technology College, Xinjiang University, Urumchi, China.
出版信息
Int J Biol Macromol. 2021 Mar 31;174:110-119. doi: 10.1016/j.ijbiomac.2021.01.165. Epub 2021 Jan 27.
To obtain porous starch granules with higher absorption capacities, three types of enzyme combinations were adopted to modify wheat and maize starches: (1) sequential α-amylase (AA) → glucoamylase (GA); (2) sequential branching enzyme (BE) → GA; and (3) sequential AA→BE→GA. The results indicated that AA→BE→GA treatment had a most optimal influence on porous starches. Compared to AA→GA and BE→GA, the mesopores in wheat starch granules treated with AA→BE→GA decreased by 52.82 and 48.70%, respectively. Conversely, the macropores increased by 216.68 and 138.18%, respectively. While for maize starch, the percentages of mesopores and macropores hardly changed after three enzyme combinations. Comparing the three enzyme treatments showed that pore volume (0.005 and 0.007 cm/g) and pore size (36.35 and 26.54 nm) were largest in the AA→BE→GA treated wheat and maize starches, respectively. Compared to the AA→GA and BE→GA, the adsorption capacities for oil, dye and heavy metal ions, wheat starch treated with AA→BE→GA increased by 46.61 and 242.33%, and 44.52 and 134.41%, and 28.83 and 271.72%, respectively. Correspondingly, that of maize starch increased by 29.71 and 133.29%, and 42.92 and 79.93%, and 28.16 and 161.43%, respectively. These results may provide a new and valuable enzyme combination for optimising porous starch granules with higher absorption capacities.
为了获得具有更高吸收能力的多孔淀粉颗粒，采用了三种酶组合来修饰小麦和玉米淀粉：（1）顺序α-淀粉酶（AA）→糖化酶（GA）；（2）顺序分支酶（BE）→GA；和（3）顺序 AA→BE→GA。结果表明，AA→BE→GA 处理对多孔淀粉的影响最为理想。与 AA→GA 和 BE→GA 相比，AA→BE→GA 处理的小麦淀粉颗粒中的中孔分别减少了 52.82%和 48.70%。相反，大孔分别增加了 216.68%和 138.18%。而对于玉米淀粉，经过三种酶组合处理后，中孔和大孔的百分比几乎没有变化。比较三种酶处理表明，AA→BE→GA 处理的小麦和玉米淀粉的孔体积（0.005 和 0.007 cm/g）和孔径（36.35 和 26.54 nm）最大。与 AA→GA 和 BE→GA 相比，AA→BE→GA 处理的小麦淀粉对油、染料和重金属离子的吸附能力分别增加了 46.61%和 242.33%、44.52%和 134.41%、28.83%和 271.72%。相应地，玉米淀粉的吸附能力分别增加了 29.71%和 133.29%、42.92%和 79.93%、28.16%和 161.43%。这些结果可能为优化具有更高吸收能力的多孔淀粉颗粒提供一种新的、有价值的酶组合。
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