Tinguely Simone M, David Arthur, Lange Anke, Tyler Charles R
University of Exeter, Biosciences, College of Life & Environmental Sciences, Exeter EX4 4QD, United Kingdom.
University of Sussex, School of Life Sciences, Brighton BN1 9QJ, United Kingdom.
Aquat Toxicol. 2021 Jan 18;232:105746. doi: 10.1016/j.aquatox.2021.105746.
The viviparous teleost Redtail Splitfin (Xenotoca eiseni) is a live bearing fish that presents a novel freshwater model for investigating the effects of maternally derived micropollutants on vulnerable early developmental life stages. Here, adult female X. eiseni were exposed to 17α-ethinyloestradiol (EE2), a potent contraceptive oestrogen, at environmentally relevant concentrations, to investigate for effects on sex partitioning and development. Pregnant and non-pregnant females were exposed for four weeks to EE2 at measured concentrations of 0.9 and 3.4 ng/L EE2 and offspring from gravid females were kept in clean water for a further four weeks. Only pregnant females were seen to respond to 3.4 ng/L EE2 with an increase in the transcription of hepatic vitellogenins (vtgA, vtgB and vtgC). Offspring of exposed mothers showed no obvious effects on somatic growth, gonadal development, sex partitioning or development. However, there was a higher rate of deformities and developmental abnormalities in offspring of EE2-exposed females. The work presented provides the foundation for the development of X. eiseni as a new freshwater model for studies on maternal transfer of chemical pollutants in live bearing animals.
胎生硬骨鱼红尾裂鳍鱼（Xenotoca eiseni）是一种胎生鱼类，为研究母体来源的微污染物对脆弱的早期发育阶段的影响提供了一种全新的淡水模型。在此，成年雌性红尾裂鳍鱼暴露于环境相关浓度的17α-乙炔雌二醇（EE2）（一种强效避孕雌激素）中，以研究其对性别分配和发育的影响。怀孕和未怀孕的雌性在测得浓度为0.9和3.4 ng/L的EE2环境中暴露四周，怀孕雌性的后代在清洁水中再饲养四周。仅观察到怀孕雌性对3.4 ng/L的EE2有反应，其肝脏卵黄蛋白原（vtgA、vtgB和vtgC）的转录增加。暴露母体的后代在体细胞生长、性腺发育、性别分配或发育方面未表现出明显影响。然而，暴露于EE2的雌性后代出现畸形和发育异常的比例更高。本文所开展的工作为将红尾裂鳍鱼开发成为研究胎生动物中化学污染物母体转移的新淡水模型奠定了基础。
