Department of Genetics, Faculty of Agriculture, Alexandria University, Alexandria, 21545, Egypt.
Department of Genetics, Faculty of Agriculture, Alexandria University, Alexandria, 21545, Egypt.
Plant Physiol Biochem. 2021 Mar;160:218-224. doi: 10.1016/j.plaphy.2021.01.024. Epub 2021 Jan 21.
Plants are constantly challenged with several biotic and abiotic stresses, and the adaptation to these stresses requires molecular and morphological changes. Epigenetic regulation provides effective control that enables plants to tolerate stress, which results in improved survivability. The distinct role of abscisic acid (ABA) in controlling numerous stress-responsive genes and enhancing respiration metabolism is well known; however, whether DNA methylation is associated with the regulation of ABA-dependent gene expression remains unclear. This study was conducted to identify the changes in DNA methylation induced by drought stress in ABA-deficient maize mutant vp10 using the amplified methylation polymorphism-polymerase chain reaction (AMP-PCR) technique. Differentially methylated DNA fragments were mapped to intragenic regions of zinc finger, amino acid catabolic enzymes, and other genes implicated in DNA repair and plant survival, in addition to several demethylated transposable elements.
植物不断受到生物和非生物胁迫的挑战，适应这些胁迫需要分子和形态的变化。表观遗传调控提供了有效的控制，使植物能够耐受应激，从而提高生存能力。脱落酸 (ABA) 在控制许多应激响应基因和增强呼吸代谢方面的独特作用是众所周知的；然而，DNA 甲基化是否与 ABA 依赖的基因表达调控有关尚不清楚。本研究采用扩增甲基化多态性-聚合酶链反应 (AMP-PCR) 技术，研究了 ABA 缺陷型玉米突变体 vp10 在干旱胁迫下 DNA 甲基化的变化。差异甲基化 DNA 片段被映射到锌指、氨基酸代谢酶等基因的基因内区域，这些基因与 DNA 修复和植物存活有关，此外还映射到一些去甲基化的转座元件。
