Yang Li, Sun Jingwen, Yang Tong, Zhang Xumei, Xu Chenghui, Wei Yaya, Li Yongshuai, Zhao Yan, Zhang Sheng, Wu Quanxin, Shi Huijun, Fu Qiang, Xia Lining
Xinjiang Key Laboratory of Herbivore Drug Research and Creation, College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, Xinjiang, China.
Front Microbiol. 2024 Nov 27;15:1458579. doi: 10.3389/fmicb.2024.1458579. eCollection 2024.
The present study aimed to investigate the therapeutic effects of berberine (BBR) on enteritis in broiler chickens and to elucidate its mechanisms of action preliminarily. Blood samples were collected from 21- to 35-day-old Sanhuang male chicks to measure immune and biochemical indicators and to calculate the organ coefficients for the liver, spleen, bursa of Fabricius, and thymus. The caecal microbiota was analysed through 16S ribosomal RNA (rRNA) gene sequencing, and transcriptome sequencing was conducted. Compared with the positive control group (S), the berberine-treated group (BS) presented increased serum immunoglobulin M (IgM) levels, serum IgG levels, and total antioxidant capacity; berberine ameliorated the increase in the thymus index caused by administration. The addition of berberine to the diet increased the abundance of beneficial bacterial genera, including and . It also decreased the abundance of harmful bacterial genera, including and . Transcriptome analysis revealed that gene expression in the S and BS groups was associated with T cell selection and B cell receptor signalling pathways, which are enriched primarily in multiple immune-related signalling pathways, including the B cell receptor signalling pathway, NF- B signalling pathway, intestinal immune network for IgA production, asthma, and African trypanosomiasis. The significantly expressed genes included ATAD5, ERP29, MGST2, PIK3CA, and HSP90AA1. The present study demonstrated that berberine has a good therapeutic effect on infection in chicks, as it inhibits the occurrence and development of -induced intestinal inflammation by regulating the balance of the gut microbiota and the expression of related genes, including ATAD5, ERP29, MGST2, PIK3CA, and HSP90AA1.
本研究旨在探讨黄连素（BBR）对肉鸡肠炎的治疗作用，并初步阐明其作用机制。采集21至35日龄的三黄雄性雏鸡的血液样本，以检测免疫和生化指标，并计算肝脏、脾脏、法氏囊和胸腺的器官系数。通过16S核糖体RNA（rRNA）基因测序分析盲肠微生物群，并进行转录组测序。与阳性对照组（S）相比，黄连素治疗组（BS）的血清免疫球蛋白M（IgM）水平、血清IgG水平和总抗氧化能力均有所提高；黄连素改善了给药引起的胸腺指数升高。在日粮中添加黄连素增加了有益细菌属的丰度，包括 和 。它还降低了有害细菌属的丰度，包括 和 。转录组分析显示，S组和BS组的基因表达与T细胞选择和B细胞受体信号通路相关，这些通路主要富集于多种免疫相关信号通路，包括B细胞受体信号通路、NF-κB信号通路、IgA产生的肠道免疫网络、哮喘和非洲锥虫病。显著表达的基因包括ATAD5、ERP29、MGST2、PIK3CA和HSP90AA1。本研究表明，黄连素对雏鸡 感染具有良好的治疗效果，因为它通过调节肠道微生物群的平衡以及包括ATAD5、ERP29、MGST2、PIK3CA和HSP90AA1在内的相关基因的表达，抑制了 诱导的肠道炎症的发生和发展。
