College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, China.
College of animal science and Technology, Hebei Agricultural University, Baoding 071001, Hebei, China.
Poult Sci. 2019 Feb 1;98(2):828-841. doi: 10.3382/ps/pey393.
A study was conducted to study the effects of glucose oxidase (GOD) supplement on the growth performance, gut function, and cecal microbiota in broiler chickens from 1 to 42 d, and further evaluate the use of GOD as an antibiotic substitution. A total of 525 1-d-old healthy Arbor Acres broilers were randomly assigned to five treatments, including control group, antibiotic growth promoters (AGP) supplement group, and three GOD supplement groups, with seven replicates per treatment and 15 birds per replicate. Growth performance, gut function including digestive ability and gut barrier, and cecal microbiota were determined. Compared with the control group, the increased daily body weight gain, improved meat quality, and enhanced digestive ability that indicated from the nutrients apparent digestibility and digestive enzymes were identified in GOD supplement groups, which could have a similar effect with the AGP supplement. The content of secreted immunoglobulin A and the transepithelial electrical resistance were also increased with the GOD supplement, which indicated an enhanced gut barrier. Additionally, 16S rRNA gene of cecal contents was sequenced by high-throughput sequencing. Sequencing data indicated that the Firmicutes phylum, Ruminococcaceae and Rikenellaceae families, Faecalibacterium genus, and F. prausnitzii species were significantly altered. Especially, combined with previous studies, our results indicated that the significantly increased F. prausnitzii, Ruminococcaceae, and Firmicutes could be involved in the effect of GOD on gut function and growth performance of broilers. Our results indicated that dietary GOD supplement could improve the growth performance of broilers in two main ways: by enhancing the digestive function of gut, which concluded from the improved nutrients apparent digestibility and digestive enzyme, and by increasing the abundance of beneficial bacterium, such as F. prausnitzii, Ruminococcaceae, and Firmicutes, which could be further served as an important regulator to improve the growth performance and the gut health.
一项研究旨在探讨葡萄糖氧化酶（GOD）补充剂对 1 至 42 日龄肉鸡生长性能、肠道功能和盲肠微生物群的影响，并进一步评估 GOD 作为抗生素替代品的应用。研究共选用了 525 只 1 日龄健康的 Arbor Acres 肉鸡，随机分为 5 个处理组，包括对照组、抗生素生长促进剂（AGP）补充组和 3 个 GOD 补充组，每个处理组设 7 个重复，每个重复 15 只鸡。测定了生长性能、肠道功能（包括消化能力和肠道屏障）和盲肠微生物群。与对照组相比，GOD 补充组肉鸡的日增重增加，肉质改善，营养物质表观消化率和消化酶活力提高，表明其具有与 AGP 补充剂相似的效果。GOD 补充剂还能提高肠道分泌型免疫球蛋白 A（sIgA）含量和跨上皮电阻（TEER），表明肠道屏障增强。此外，还通过高通量测序对盲肠内容物的 16S rRNA 基因进行了测序。测序数据表明，厚壁菌门、瘤胃科和真杆菌科、粪杆菌属和普雷沃氏菌属 F. prausnitzii 种的含量发生了显著变化。特别是，结合以前的研究，我们的结果表明，显著增加的 F. prausnitzii、瘤胃科和厚壁菌门可能参与了 GOD 对肉鸡肠道功能和生长性能的影响。我们的研究结果表明，日粮 GOD 补充剂可以通过两种主要方式提高肉鸡的生长性能：一是通过提高肠道的消化功能，这可以从提高营养物质的表观消化率和消化酶活力得到结论；二是通过增加有益菌的丰度，如 F. prausnitzii、瘤胃科和厚壁菌门，这些有益菌可以进一步作为重要的调节剂，改善生长性能和肠道健康。
