Department of Oral and Maxillofacial Surgery, Program of Dentistry, Graduate School of Biomedical and Health Sciences, Hiroshima University.
Department of Dentistry and Oral Surgery, Shinshu University Hospital.
Dent Mater J. 2021 May 29;40(3):712-718. doi: 10.4012/dmj.2020-224. Epub 2021 Jan 30.
The purpose of this study was to evaluate the surface modification of calcium ions on roughened titanium as a surface treatment of dental implants for cell attachment, growth, and initial bacterial adhesion. When a surface-roughened, pure titanium disk was immersed in a calcium chloride solution (100 mM) containing 20 ppm ozone for 24 h at 25ºC, calcium was detected on the surface by X-ray photoelectron spectroscopy. The calcium-modified, roughened titanium disk had a significantly greater concentration of the initially adhered cells as well as cells cultured over 7 days compared with titanium disks without surface modification. Furthermore, the initial bacterial adhesion on the calcium-ozone treated titanium disk was statistically less than on a pure titanium disk or titanium disk treated without ozone. Dissolved ozone was useful for modifying the surface of roughened titanium with calcium ions and the surface modification may be applicable for dental implants.
本研究旨在评估钙离子对粗糙化钛表面的修饰作用，将其作为一种改善牙科植入物表面细胞附着、生长和初始细菌黏附的处理方法。当表面粗糙化的纯钛盘在 25°C 下于含有 20ppm 臭氧的 100mM 氯化钙溶液中浸泡 24 小时时，通过 X 射线光电子能谱法在表面检测到钙。与未经表面修饰的钛盘相比，钙修饰的粗糙钛盘表面初始黏附的细胞以及培养 7 天后的细胞浓度显著增加。此外，经钙-臭氧处理的钛盘上初始细菌黏附量明显少于纯钛盘或未经臭氧处理的钛盘。溶解臭氧可有效对粗糙化钛表面进行钙离子修饰，表面修饰可能适用于牙科植入物。
