Molecular Imaging and NanoBioTechnology, University of Bordeaux, Pessac, France.
Institut Universitaire de France, Paris, France;
J Extracell Vesicles. 2015 Dec 23;4:29509. doi: 10.3402/jev.v4.29509. eCollection 2015.
Plasma and other body fluids contain cell-derived extracellular vesicles (EVs), which participate in physiopathological processes and have potential biomedical applications. In order to isolate, concentrate and purify EVs, high-speed centrifugation is often used. We show here, using electron microscopy, receptor-specific gold labelling and flow cytometry, that high-speed centrifugation induces the formation of EV aggregates composed of a mixture of EVs of various phenotypes and morphologies. The presence of aggregates made of EVs of different phenotypes may lead to erroneous interpretation concerning the existence of EVs harbouring surface antigens from different cell origins.
血浆和其他体液中含有细胞衍生的细胞外囊泡 (EVs)，它们参与生理病理过程，并具有潜在的生物医学应用。为了分离、浓缩和纯化 EVs，通常使用高速离心。我们在这里通过电子显微镜、受体特异性金标记和流式细胞术显示，高速离心会诱导由各种表型和形态的 EV 混合物组成的 EV 聚集物的形成。由不同表型的 EV 组成的聚集物的存在可能导致关于存在携带来自不同细胞来源的表面抗原的 EV 的错误解释。
