Bu Haidong, Yu Wenquan, Yuan Hui, Yue Pengtao, Wei Yun, Wang Aide
Key Laboratory of Fruit Postharvest Biology, College of Horticulture, Shenyang Agricultural University, Shenyang, China.
Mudanjiang Branch of Heilongjiang Academy of Agricultural Sciences, Mudanjiang, China.
Front Plant Sci. 2020 Dec 3;11:592540. doi: 10.3389/fpls.2020.592540. eCollection 2020.
Fruit size is an important economic trait that is controlled by multiple genes. However, the regulatory mechanism for fruit size remains poorly understood. A bud sport variety of "Longfeng" (LF) apple () was identified and named "Grand Longfeng" (GLF). The fruit size of GLF is larger than that of LF, and both varieties are diploid. We found that the cell size in GLF fruit was larger than that of LF. Then, we compared the fruit transcriptomes of the two varieties using RNA-Seq technology. A total of 1166 differentially expressed genes (DEGs) were detected between GLF and LF fruits. The KEGG analysis revealed that the phytohormone pathway was the most enriched, in which most of the DEGs were related to auxin signaling. Moreover, the endogenous auxin levels of GLF fruit were higher than those of LF. The expressions of auxin synthetic genes, including and , were higher in GLF fruit than LF. Collectively, our findings suggest that auxin plays an important role in fruit size development.
果实大小是一个受多基因控制的重要经济性状。然而，果实大小的调控机制仍知之甚少。鉴定出一个“龙凤”（LF）苹果的芽变品种，并命名为“大龙凤”（GLF）。GLF的果实大小大于LF，且两个品种均为二倍体。我们发现GLF果实中的细胞大小大于LF。然后，我们使用RNA-Seq技术比较了两个品种的果实转录组。在GLF和LF果实之间共检测到1166个差异表达基因（DEG）。KEGG分析表明，植物激素途径富集程度最高，其中大多数DEG与生长素信号传导有关。此外，GLF果实的内源生长素水平高于LF。包括 和 在内的生长素合成基因在GLF果实中的表达高于LF。总的来说，我们的研究结果表明生长素在果实大小发育中起重要作用。
