Asiedu-Gyekye I J, Borovskaya T G, Poluektova M E, Vychuzhanina А V, Shchemerovа Y А, Kamalova S I, Grgoreva V A, Amoateng P, Kukuia K E, Kwapong A A, Allotey Babington L, Amponsah S K, N'guessan B B
Department of Pharmacology and Toxicology, School of Pharmacy, College of Health Sciences, University of Ghana, Legon, Accra, Ghana.
Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
J Toxicol. 2021 Jan 12;2021:6114672. doi: 10.1155/2021/6114672. eCollection 2021.
Unsweetened natural cocoa (UNCP) was evaluated for reproductive toxicity in rats. A preliminary genotoxic potential was evaluated by the DNA comet assay test using C57Bl/6 mice. Both therapeutic dose (TD; 900 mg/kg) and high dose (HD; 9000 mg/kg) of UNCP were used. White Wistar rats were used in two experimental groups. The females received UNCP 15 days before crossing with untreated males. The males received UNCP for 48 days before mating with untreated females. Subacute toxicity was observed during a 14-day oral administration of UNCP. Results show that a high tail DNA% was observed with methyl mesylate administration in all tissues analysed. The lowest tail DNA% value was observed in the liver (1.64 ± 0.26) and kidney (1.63 ± 0.30) during UNCP (TD) administration. UNCP did not induce observable physical congenital malformations on the pubs of treated female and male rats, lacks genotoxic potential, and did not adversely affect pregnancy index, pub weights, and survival index, but UNCP exhibited proimplantation potential ( > 0.05).
对未加糖的天然可可（UNCP）进行了大鼠生殖毒性评估。使用C57Bl/6小鼠通过DNA彗星试验对其初步遗传毒性潜力进行了评估。使用了UNCP的治疗剂量（TD；900毫克/千克）和高剂量（HD；9000毫克/千克）。两个实验组使用了白色Wistar大鼠。雌性大鼠在与未处理的雄性大鼠交配前15天接受UNCP。雄性大鼠在与未处理的雌性大鼠交配前48天接受UNCP。在UNCP口服给药14天期间观察到亚急性毒性。结果表明，在所有分析的组织中，甲磺酸甲酯给药后观察到高尾DNA%。在UNCP（TD）给药期间，肝脏（1.64±0.26）和肾脏（1.63±0.30）中观察到最低的尾DNA%值。UNCP未在经处理的雌性和雄性大鼠后代中诱导可观察到的身体先天性畸形，缺乏遗传毒性潜力，并且未对妊娠指数、幼崽体重和存活指数产生不利影响，但UNCP表现出着床前潜力（>0.05）。
