类器官在胆管癌和胆囊癌中的应用及研究进展
Application and research progress of organoids in cholangiocarcinoma and gallbladder carcinoma.
作者信息
Huang Yun-Peng, Liu Kai, Wang Yong-Xiang, Yang Yang, Xiong Li, Zhang Zi-Jian, Wen Yu
机构信息
Department of General Surgery, The Second Xiangya Hospital of Central South University Changsha 410011, Hunan Province, China.
出版信息
Am J Cancer Res. 2021 Jan 1;11(1):31-42. eCollection 2021.
Both cholangiocarcinoma (CCA) and gallbladder carcinoma (GBC) are belong to biliary tract carcinomas (BTCs) with a high degree of malignancy and a poor prognosis. Therefore, an model is urgently needed to increase our understanding of the pathogenesis of BTCs. Tumor organoids are a novel three-dimensional (3D) culture technology that utilizes samples from removed tumors. Therefore, it can maintain the histological features, expression profiles and marker expression of the parental tissues. Recently, with the widespread use of this technique, increasing research is beginning to use organoid to study the cellular metabolism, pathogenesis, chemotherapy resistance, and new therapy methods of BTCs. In this review, we will discuss the advantages and disadvantages of BTC organoids compared with other cell culture techniques. In addition, the construction methods, research directions and current limitations of BTC organoids will be described.
胆管癌（CCA）和胆囊癌（GBC）均属于恶性程度高、预后差的胆道癌（BTC）。因此，迫切需要一个模型来增进我们对BTC发病机制的理解。肿瘤类器官是一种利用切除肿瘤样本的新型三维（3D）培养技术。因此，它可以维持亲代组织的组织学特征、表达谱和标志物表达。近年来，随着这项技术的广泛应用，越来越多的研究开始使用类器官来研究BTC的细胞代谢、发病机制、化疗耐药性和新的治疗方法。在这篇综述中，我们将讨论BTC类器官与其他细胞培养技术相比的优缺点。此外，还将描述BTC类器官的构建方法、研究方向和当前局限性。
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