利用四足点扩散函数在大轴向范围内进行精确的三维无扫描多粒子跟踪。

Shechtman Yoav, Weiss Lucien E, Backer Adam S, Sahl Steffen J, Moerner W E

†Department of Chemistry, Stanford University, 375 North-South Mall, Stanford, California 94305, United States.

‡Institute for Computational and Mathematical Engineering, 475 Via Ortega, Stanford, California 94305, United States.

Nano Lett. 2015 Jun 10;15(6):4194-9. doi: 10.1021/acs.nanolett.5b01396. Epub 2015 May 18.

We employ a novel framework for information-optimal microscopy to design a family of point spread functions (PSFs), the Tetrapod PSFs, which enable high-precision localization of nanoscale emitters in three dimensions over customizable axial (z) ranges of up to 20 μm with a high numerical aperture objective lens. To illustrate, we perform flow profiling in a microfluidic channel and show scan-free tracking of single quantum-dot-labeled phospholipid molecules on the surface of living, thick mammalian cells.

我们采用一种用于信息最优显微镜的新型框架来设计一族点扩散函数（PSF），即四足动物PSF，它能够使用高数值孔径物镜在高达20μm的可定制轴向（z）范围内对三维纳米级发射器进行高精度定位。为了说明这一点，我们在微流控通道中进行流动分析，并展示了对活的厚哺乳动物细胞表面上单量子点标记的磷脂分子的无扫描跟踪。