Taniguchi Maria, Nambu Minori, Katakura Yoshio, Yamasaki-Yashiki Shino
Chemistry, Materials and Bioengineering Major, Graduate School of Science and Engineering, Kansai University, 3-3-35 Yamate-cho, Suita, Osaka 564-8680, Japan.
Department of Life Science and Biotechnology, Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamate-cho, Suita, Osaka 564-8680, Japan.
Biosci Microbiota Food Health. 2021;40(1):59-64. doi: 10.12938/bmfh.2020-003. Epub 2020 Oct 3.
Adherence of probiotics to dietary fibers present in the intestinal tract may affect adhesion to intestinal epithelial cells. The properties of the adhesion of bifidobacteria to mucin or epithelial cells have been well studied; however, adhesion of bifidobacteria to dietary fiber has not been investigated. The adhesion ratio of six strains to cellulose and chitin was examined; among the strains, subsp. JCM 10602 showed high adherence to both cellulose and chitin, and two strains showed high adherence to only chitin. The ratios of adhesion of to cellulose and chitin were positively and negatively correlated with ionic strength, respectively. These data suggest that hydrophobic and electrostatic interactions are involved in the adhesion to cellulose and chitin, respectively. The adhesion ratios of the cells in the late logarithmic phase to cellulose and chitin decreased by approximately 40% and 70% of the cells in the early logarithmic phase, respectively. Furthermore, the adhesion ratio to cellulose decreased with increasing bile concentration regardless of the culture phase of the cells. On the other hand, the adhesion ratio to chitin of cells in the early logarithmic phase decreased with increasing bile concentration; however, that of cells in the late logarithmic phase increased slightly, suggesting that adhesins differ depending on the culture phase. Our results indicated the importance of considering adhesion to both dietary fibers and the intestinal mucosa when using bifidobacteria as probiotics.
益生菌对肠道中膳食纤维的黏附可能会影响其对肠道上皮细胞的黏附。双歧杆菌对黏蛋白或上皮细胞的黏附特性已得到充分研究；然而，双歧杆菌对膳食纤维的黏附尚未被研究。检测了6株菌对纤维素和几丁质的黏附率；其中，亚种JCM 10602对纤维素和几丁质均表现出高黏附性，另外两株菌仅对几丁质表现出高黏附性。对纤维素和几丁质的黏附率分别与离子强度呈正相关和负相关。这些数据表明，疏水相互作用和静电相互作用分别参与了对纤维素和几丁质的黏附。对数生长后期细胞对纤维素和几丁质的黏附率分别比对数生长前期细胞降低了约40%和70%。此外，无论细胞处于何种培养阶段，对纤维素的黏附率均随胆汁浓度的增加而降低。另一方面，对数生长前期细胞对几丁质的黏附率随胆汁浓度的增加而降低；然而，对数生长后期细胞的黏附率略有增加，这表明黏附素因培养阶段而异。我们的结果表明，在将双歧杆菌用作益生菌时，考虑其对膳食纤维和肠黏膜的黏附具有重要意义。
