59588Umeå Center for Molecular Medicine, Umeå University, Umeå, Sweden.
Department of Integrative Medical Biology, Umeå University, Umeå, Sweden.
Cell Transplant. 2021 Jan-Dec;30:963689720988245. doi: 10.1177/0963689720988245.
Spinal cord injury results in irreversible tissue damage and permanent sensorimotor impairment. The development of novel therapeutic strategies that improve the life quality of affected individuals is therefore of paramount importance. Cell transplantation is a promising approach for spinal cord injury treatment and the present study assesses the efficacy of human embryonic stem cell-derived neural crest cells as preclinical cell-based therapy candidates. The differentiated neural crest cells exhibited characteristic molecular signatures and produced a range of biologically active trophic factors that stimulated neurite outgrowth of rat primary dorsal root ganglia neurons. Transplantation of the neural crest cells into both acute and chronic rat cervical spinal cord injury models promoted remodeling of descending raphespinal projections and contributed to the partial recovery of forelimb motor function. The results achieved in this proof-of-concept study demonstrates that human embryonic stem cell-derived neural crest cells warrant further investigation as cell-based therapy candidates for the treatment of spinal cord injury.
脊髓损伤导致不可逆转的组织损伤和永久性感觉运动功能障碍。因此，开发能够提高患者生活质量的新型治疗策略至关重要。细胞移植是治疗脊髓损伤的一种有前途的方法，本研究评估了人胚胎干细胞衍生的神经嵴细胞作为临床前基于细胞的治疗候选物的疗效。分化的神经嵴细胞表现出特征性的分子特征，并产生一系列生物活性的营养因子，刺激大鼠原代背根神经节神经元的轴突生长。将神经嵴细胞移植到急性和慢性大鼠颈脊髓损伤模型中，促进了下行中缝脊髓投射的重塑，并有助于前肢运动功能的部分恢复。这项概念验证研究的结果表明，人胚胎干细胞衍生的神经嵴细胞作为脊髓损伤治疗的细胞治疗候选物值得进一步研究。
