Department of Biomedical Engineering, Southern University of Science and Technology, No. 1088 Xueyuan Road, Nanshan District, Shenzhen, Guangdong 518055, P.R. China.
Nano Lett. 2021 Feb 10;21(3):1335-1344. doi: 10.1021/acs.nanolett.0c04053. Epub 2021 Feb 1.
Targeted delivery of therapeutic molecules using nanomaterials is desired to elicit specific responses toward diseases. Such an integrated synthesis of functional material using a microfluidic approach is a great challenge. Functional metal organic frameworks (MOFs) with unique structural diversity possess a complicated synthesis procedure thereby requiring a modest, straightforward approach to synthesize size-controllable MOFs. Here, we develop an integrated microfluidic chip to synthesize the aptamer-modified biozeolitic imidazolate framework (BioZIF-8) to target the lymph node and tumor. The first stage of the microfluidic chip forms the ZIF-8 encapsulating biomolecules (bovine serum albumin, small interfering ribonucleic acid, and doxorubicin). The second stage modifies the surface of BioZIF-8 with the aptamer. Our approach reduces the overall synthesis time (∼3 mg/10 min against 15 h for the conventional two-step method) and encapsulates a higher number of biomolecules. The microfluidic approach realizes the rapid and fine-tuned synthesis of functional MOFs integrated into one-step.
利用纳米材料靶向递送达药物分子是诱发针对疾病的特定反应的理想方法。使用微流控方法对功能材料进行集成合成是一个巨大的挑战。具有独特结构多样性的功能金属有机骨架（MOFs）具有复杂的合成程序，因此需要一种简单、直接的方法来合成尺寸可控的 MOFs。在这里，我们开发了一种集成微流控芯片来合成适体修饰的生物沸石咪唑酯骨架（BioZIF-8），以靶向淋巴结和肿瘤。微流控芯片的第一阶段形成了包封生物分子（牛血清白蛋白、小干扰核糖核酸和阿霉素）的 ZIF-8。第二阶段用适体修饰 BioZIF-8 的表面。我们的方法缩短了总合成时间（与传统的两步法相比，约为 3 毫克/10 分钟，而不是 15 小时），并封装了更多数量的生物分子。微流控方法实现了功能 MOFs 的快速和精细合成，集成到一步中。
