基于生物纳米复合材料的高灵敏度无标记电化学免疫传感器在子宫内膜异位症诊断中的应用。
Bio-nanocomposite based highly sensitive and label-free electrochemical immunosensor for endometriosis diagnostics application.
机构信息
Department of Biotechnology, National Institute of Technology, Papum Pare 791112, Arunchal Pradesh, India.
Department of Biotechnology, National Institute of Technology, Papum Pare 791112, Arunchal Pradesh, India; Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei 10608, Taiwan.
出版信息
Bioelectrochemistry. 2021 Jun;139:107740. doi: 10.1016/j.bioelechem.2021.107740. Epub 2021 Jan 11.
In this research, for the first time, a bio-nanocomposites based highly sensitive and label-free electrochemical immunosensor is reported with the aim of endometriosis diagnostics application. Multiwalled carbon nanotube and magnetite nanoparticle (MWCNT-FeO) was dispersed in chitosan (CS) to fabricate a bio-nanocomposite to immobilize very monoclonal specific antibody (via cross-linking using glutaraldehyde) for selective electrochemical immuno-sensing of carbohydrate antigen 19-9 (CA19-9), a potential biomarker for endometriosis diagnostics. Well-characterized Anti-Abs/CS-MWCNT-FeO immune-electrode fabricated on glassy carbon electrode (GCE) successfully detect CA 19-9 and exhibited a high sensitivity as (2.55 µA pg cm), a detection limit of 0.163 pg mL, detection range from 1.0 pg mL to 100 ng mL. Our fabricated electrochemical Abs/CS-MWCNT-FeO immunosensor performed CA19-9 sensing in physiological range and at a very level which suggest it application for early-stage diagnostics, diseases monitoring, and optimization of therapy. To claim the clinical application, our sensor was tested using real samples and sensing performance was validated using enzyme-linked immune-sorbent assay (ELISA). The results of the studies projected Abs/CS-MWCNT-FeO electrochemical CA19-9 immunosensor as a potential and affordable alternate of conventional techniques like ELISA. We believe that our fabricated sensor can be the plane of a disease's management program due to affordable, rapid, label-free, and sensitive detection of a targeted biomarker.
在这项研究中，首次报道了一种基于生物纳米复合材料的高灵敏度和无标记电化学免疫传感器，旨在应用于子宫内膜异位症的诊断。多壁碳纳米管和磁铁矿纳米粒子（MWCNT-FeO）分散在壳聚糖（CS）中，以制备生物纳米复合材料，用于通过戊二醛交联固定非常单克隆特异性抗体，用于选择性电化学免疫检测糖抗原 19-9（CA19-9），CA19-9 是子宫内膜异位症诊断的潜在生物标志物。在玻碳电极（GCE）上成功制备了基于抗体制备的、具有良好特征的 Anti-Abs/CS-MWCNT-FeO 免疫电极，该电极可用于检测 CA 19-9，具有较高的灵敏度（2.55 µA pg cm），检测限为 0.163 pg mL，检测范围为 1.0 pg mL 至 100 ng mL。我们制备的电化学 Abs/CS-MWCNT-FeO 免疫传感器在生理范围内对 CA19-9 进行了传感，并且检测水平非常低，这表明它可用于早期诊断、疾病监测和治疗优化。为了声称临床应用，我们使用实际样本对传感器进行了测试，并使用酶联免疫吸附测定（ELISA）验证了传感器的性能。研究结果表明，Abs/CS-MWCNT-FeO 电化学 CA19-9 免疫传感器作为一种有前途的替代传统技术（如 ELISA）的方法，具有经济实惠、快速、无标记和对目标生物标志物的敏感检测等优点。我们相信，由于能够以经济实惠、快速、无标记和敏感的方式检测靶向生物标志物，我们制备的传感器可以成为疾病管理计划的一个平台。
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