State Key Laboratory of Food Science and Technology, School of Food Science and Technology, National Engineering Research Center for Functional Food, Synergetic Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, National Engineering Research Center for Functional Food, Synergetic Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.
Bioelectrochemistry. 2021 Jun;139:107743. doi: 10.1016/j.bioelechem.2021.107743. Epub 2021 Jan 18.
A "honeycomb" electrochemical biosensor based on 3D printing was developed to noninvasively monitor the viability of 3D cells and evaluate the individual or combined toxicity of deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-ADON), and 15-acetyldeoxynivalenol (15-ADON). Carbon nanofiber (CN)/gelatin methacryloyl (GelMA) conductive composite hydrogel with strong processability was printed on 8-channel screen-printed carbon electrodes (SPCEs) to maintain cell viability and form tight cell-to-cell contacts. A "3D honeycomb" printing infill pattern was selected in the construction of the biosensors to improve conductivity. Based on 3D printing technology, the electrochemical biosensor can prevent manual error and provide for high-throughput detection. Electrochemical impedance spectroscopy (EIS) was used to evaluate mycotoxin toxicity. The EIS response decreased with the concentration of DON, 3-ADON and 15-ADON in the range of 0.1-10, 0.05-100, and 0.1-10 μg/mL, respectively, with a limit of detection of 0.07, 0.10 and 0.06 μg/mL, respectively. Mycotoxin interactions were analyzed using the isobologram-combination index (CI) method. The electrochemical cytotoxicity evaluation result was confirmed by biological assays. Therefore, a novel method for evaluating the combined toxicity of mycotoxins is proposed, which exhibits potential for application to food safety and evaluation.
基于 3D 打印技术的“蜂窝状”电化学生物传感器用于非侵入式监测 3D 细胞活力，并评估脱氧雪腐镰刀菌烯醇（DON）、3-乙酰脱氧雪腐镰刀菌烯醇（3-ADON）和 15-乙酰脱氧雪腐镰刀菌烯醇（15-ADON）的单独或联合毒性。打印在 8 通道丝网印刷碳电极（SPCE）上的具有强加工性能的碳纳米纤维（CN）/甲基丙烯酰化明胶（GelMA）导电复合水凝胶，可维持细胞活力并形成紧密的细胞间接触。在生物传感器的构建中选择了“3D 蜂窝状”打印填充图案，以提高导电性。基于 3D 打印技术，电化学生物传感器可以防止人为错误并实现高通量检测。电化学阻抗谱（EIS）用于评估真菌毒素毒性。EIS 响应随 DON、3-ADON 和 15-ADON 的浓度在 0.1-10、0.05-100 和 0.1-10μg/mL 的范围内降低，检出限分别为 0.07、0.10 和 0.06μg/mL。采用等辐射图-组合指数（CI）法分析真菌毒素相互作用。生物测定法证实了电化学细胞毒性评估结果。因此，提出了一种评估真菌毒素联合毒性的新方法，该方法具有应用于食品安全和评估的潜力。
