Institute of Advanced Synthesis, School of Chemistry and Molecular Engineering, Jiangsu National Synergetic Innovation Center for Advanced Materials, Nanjing Tech University, Nanjing 211816, China.
Institute of Advanced Synthesis, School of Chemistry and Molecular Engineering, Jiangsu National Synergetic Innovation Center for Advanced Materials, Nanjing Tech University, Nanjing 211816, China.
J Colloid Interface Sci. 2021 May 15;590:125-133. doi: 10.1016/j.jcis.2021.01.036. Epub 2021 Jan 18.
Surface enhanced Raman scattering (SERS)-based nanoprobes have been used as well-established analytical tools enabling single-molecule detection. In this work, we report a facile method to decorate sea urchin-like gold nanoparticles (SUGNPs) on the surface of PMMA/P4VP nanofibers. Firstly, PMMA/P4VP nanofibers within the submicrometer size range were prepared by applying the electrospinning technique. Then, the incorporation of SUGNPs on the surface of PMMA/P4VP nanofiber was achieved by immersing PMMA/P4VP nanofiber into freshly prepared SUGNP aqueous solution through the specific Au-N interactions. The as-fabricated SUGNP-coated PMMA/P4VP nanofibers exhibited good sensitivity and reproducibility in SERS measurements with the relative standard deviation down to 6.6%, by employing 4-mercaptobenzoic acid as a probe molecule with 30 min of soaking time. Hence, we envisage that the SUGNP-coated PMMA/P4VP nanofibers can act as efficient and stable SERS substrates for potential applications in molecular detection as well as chemical and biological analysis.
基于表面增强拉曼散射（SERS）的纳米探针已被用作成熟的分析工具，能够实现单分子检测。在这项工作中，我们报告了一种在 PMMA/P4VP 纳米纤维表面修饰海胆状金纳米颗粒（SUGNP）的简便方法。首先，通过应用静电纺丝技术制备了亚微米尺寸范围内的 PMMA/P4VP 纳米纤维。然后，通过将 PMMA/P4VP 纳米纤维浸入新制备的 SUGNP 水溶液中，利用特定的 Au-N 相互作用，将 SUGNP 结合到 PMMA/P4VP 纳米纤维的表面上。通过使用 4-巯基苯甲酸作为探针分子，在 30 分钟的浸泡时间内，所制备的 SUGNP 涂覆的 PMMA/P4VP 纳米纤维在 SERS 测量中表现出良好的灵敏度和重现性，相对标准偏差低至 6.6%。因此，我们设想 SUGNP 涂覆的 PMMA/P4VP 纳米纤维可以作为高效稳定的 SERS 基底，用于分子检测以及化学和生物分析等潜在应用。
