Iio T, Yoden K
Showa College of Pharmaceutical Sciences, Tokyo, Japan.
Lipids. 1988 Jan;23(1):65-7. doi: 10.1007/BF02535307.
The hydroperoxides of methyl linoleate, 1-palmitoyl-2-linoleoyl-phosphatidylcholine and trilinolein each produced similar fluorescent substances through reaction with amino compounds after decomposition by heme methyl ester. Fluorescent substances formed from methyl linoleate with 1-aminopentane revealed characteristic fluorescence peaks on HPLC, while those obtained from 1-palmitoyl-2-linoleoyl-phosphatidylcholine and trilinolein were not eluted under the same conditions. However, when both of these fluorescent substances were transesterified to methyl ester, the same fluorescence peaks were observed. This result suggests that fluorescent substances formed from oxidized membrane lipids with amino compounds remain attached to phospholipids without being released from their glycerol backbone.
亚油酸甲酯、1-棕榈酰-2-亚油酰磷脂酰胆碱和三油精的氢过氧化物在经血红素甲酯分解后，各自与氨基化合物反应生成了类似的荧光物质。亚油酸甲酯与1-氨基戊烷反应生成的荧光物质在高效液相色谱上显示出特征荧光峰，而在相同条件下，1-棕榈酰-2-亚油酰磷脂酰胆碱和三油精生成的荧光物质未被洗脱。然而，当这两种荧光物质都被酯交换为甲酯时，观察到了相同的荧光峰。这一结果表明，由氧化膜脂与氨基化合物形成的荧光物质仍附着在磷脂上，未从其甘油主链上释放出来。
