Iio T, Yoden K
Showa College of Pharmaceutical Sciences, Tokyo, Japan.
Lipids. 1988 Oct;23(10):937-41. doi: 10.1007/BF02536340.
Phosphatidylcholine hydroperoxide produced a fluorescent substance (FS-III) through reaction with 1-amino-pentane after preincubation with heme methyl ester as a model system. The FS-III was retained at the 2-position of the glycerol backbone of phosphatidylcholine without breakdown into low molecular weight compounds. Phosphatidylcholine oxidized by catalysis with ferrous ion and ascorbic acid also produced the same fluorescent substance (FS-III). Phospholipase A2 specifically hydrolyzed the FS-III attached to the phospholipid, making it possible to elute the same fluorescent substance (FS-II) as that obtained from oxidized methyl linoleate. The release of FS-II by hydrolysis of FS-III attached to phospholipid increased with greater phospholipase A2 activity. It is suggested that, with aging, the accumulation of fluorescent lipofuscin pigments in biomembranes may be related to changes in the peroxidized phospholipid content and that phospholipase A2 may play a role in decreasing the formation and accumulation of fluorescent phospholipids in biomembranes.
以血红素甲酯作为模型系统进行预孵育后，过氧化氢磷脂酰胆碱与1-氨基戊烷反应生成了一种荧光物质（FS-III）。FS-III保留在磷脂酰胆碱甘油主链的2位，没有分解成低分子量化合物。经亚铁离子和抗坏血酸催化氧化的磷脂酰胆碱也产生了相同的荧光物质（FS-III）。磷脂酶A2特异性地水解附着在磷脂上的FS-III，从而有可能洗脱与氧化亚油酸甲酯得到的相同荧光物质（FS-II）。随着磷脂酶A2活性的增强，附着在磷脂上的FS-III经水解释放出的FS-II增多。这表明，随着衰老，生物膜中荧光脂褐素色素的积累可能与过氧化磷脂含量的变化有关，并且磷脂酶A2可能在减少生物膜中荧光磷脂的形成和积累方面发挥作用。
