Université de Pau et des Pays de l'Adour, UPPA/E2S, IPREM UMR CNRS 5254, Bat. IBEAS, Pau, France.
Helmholtz Centre for Environmental Research - UFZ, Department of Environmental Microbiology, Leipzig, 04318, Germany.
Mar Pollut Bull. 2021 Mar;164:112082. doi: 10.1016/j.marpolbul.2021.112082. Epub 2021 Jan 30.
The cultivable fungal diversity from PAH-contaminated sediments was examined for the tolerance to polycyclic aromatic hydrocarbon (PAH). The 85 fungal strains, isolated in non-selective media, revealed a large diversity by ribosomal internal transcribed spacer (ITS) sequencing, even including possible new species. Most strains (64%) exhibited PAH-tolerance, indicating that sediments retain diverse cultivable PAH-tolerant fungi. The PAH-tolerance was linked neither to a specific taxon nor to the peroxidase genes (LiP, MnP and Lac). Examining the PAH-removal (degradation and/or sorption), Alternaria destruens F10.81 showed the best capacity with above 80% removal for phenanthrene, pyrene and fluoranthene, and around 65% for benzo[a]pyrene. A. destruens F10.81 internalized pyrene homogenously into the hyphae that contrasted with Fusarium pseudoygamai F5.76 in which PAH-vacuoles were observed but PAH removal was below 20%. Thus, our study paves the way for the exploitation of fungi in remediation strategies to mitigate the effect of PAH in coastal marine sediments.
从多环芳烃污染沉积物中可培养的真菌多样性进行了研究，以考察其对多环芳烃（PAH）的耐受性。通过核糖体内部转录间隔区（ITS）测序，从非选择性培养基中分离出的 85 株真菌菌株显示出丰富的多样性，甚至包括可能的新物种。大多数菌株（64%）表现出对 PAH 的耐受性，表明沉积物中保留了具有丰富可培养 PAH 耐受性的真菌。PAH 耐受性既与特定的分类群无关，也与过氧化物酶基因（LiP、MnP 和 Lac）无关。考察了多环芳烃的去除（降解和/或吸附），发现Alternaria destruens F10.81 对菲、芘和荧蒽的去除率超过 80%，对苯并[a]芘的去除率约为 65%。Alternaria destruens F10.81 将芘均匀地内化到菌丝中，这与 Fusarium pseudoygamai F5.76 形成对比，后者观察到 PAH 液泡，但 PAH 的去除率低于 20%。因此，我们的研究为利用真菌在修复策略中减轻沿海海洋沉积物中 PAH 的影响铺平了道路。
