Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of Korea.
Department of Aquaculture, Korea National College of Agriculture and Fisheries, Jeonju 54874, Republic of Korea.
Int J Syst Evol Microbiol. 2019 Dec;69(12):3851-3856. doi: 10.1099/ijsem.0.003696.
This study describes the biochemical and phylogenetic characteristics of a Gram-negative strain, SNU WT1, isolated from rainbow trout kidney. The 16S rRNA gene sequencing indicated that strain SNU WT1 was highly similar to CCOS 864 and closely related to other -related strains. Multilocus sequence analysis of concatenated partial and sequences revealed that strain SNU WT1 was distinct from -related strains and formed a separate clade. The average nucleotide identity and Genome-to-Genome Distance Calculator values were 90.19 and 41.7 %with its closest relative CCOS 864; however, it was phenotypically distinct from CCOS 864 with respect to arginine dihydrolase, glucose fermentation, aesculin hydrolysis and -acetyl-glucosamine assimilation. The major polar lipid of the strain was phosphatidylethanolamine and the major quinone was Q-9. The genome of strain SNU WT1 had 5 685 196 bp with a G+C content of 61.83 mol%. We describe a novel species of genus , for which the name has been proposed, with SNU WT1 (=KCTC 72265=JCM 33436) as the type strain.
本研究描述了一株从虹鳟肾脏中分离得到的革兰氏阴性菌株 SNU WT1 的生化和系统发育特征。16S rRNA 基因测序表明,菌株 SNU WT1 与 CCOS 864 高度相似,与其他相关菌株密切相关。部分和串联序列的多位点序列分析显示,菌株 SNU WT1 与相关菌株明显不同,并形成一个单独的分支。与最接近的亲缘关系菌株 CCOS 864 的平均核苷酸同一性和基因组到基因组距离计算器值分别为 90.19%和 41.7%;然而,与 CCOS 864 相比,菌株 SNU WT1 在精氨酸二氢酶、葡萄糖发酵、马栗树皮苷水解和乙酰 -D-葡萄糖胺同化方面表现出明显的表型差异。该菌株的主要极性脂是磷脂酰乙醇胺,主要醌是 Q-9。菌株 SNU WT1 的基因组有 5685196bp,G+C 含量为 61.83mol%。我们描述了一个新的属种,建议将其命名为,其模式菌株为 SNU WT1(=KCTC 72265=JCM 33436)。