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CRISPR系统在植物基因组编辑中的应用:迈向农业新机遇

Use of CRISPR systems in plant genome editing: toward new opportunities in agriculture.

作者信息

Ricroch Agnès, Clairand Pauline, Harwood Wendy

机构信息

AgroParisTech, 16 rue Claude Bernard, Paris F-75231, France.

Université de Paris-Sud, Faculté Jean-Monnet, Collège d'Etudes Interdisciplinaires, 54, Boulevard Desgranges, Sceaux F-92330, France.

出版信息

Emerg Top Life Sci. 2017 Nov 10;1(2):169-182. doi: 10.1042/ETLS20170085.

Abstract

Initially discovered in bacteria and archaea, CRISPR-Cas9 is an adaptive immune system found in prokaryotes. In 2012, scientists found a way to use it as a genome editing tool. In 2013, its application in plants was successfully achieved. This breakthrough has opened up many new opportunities for researchers, including the opportunity to gain a better understanding of plant biological systems more quickly. The present study reviews agricultural applications related to the use of CRISPR systems in plants from 52 peer-reviewed articles published since 2014. Based on this literature review, the main use of CRISPR systems is to achieve improved yield performance, biofortification, biotic and abiotic stress tolerance, with rice (Oryza sativa) being the most studied crop.

摘要

CRISPR-Cas9最初是在细菌和古生菌中发现的,是一种存在于原核生物中的适应性免疫系统。2012年,科学家们找到了将其用作基因组编辑工具的方法。2013年,它在植物中的应用成功实现。这一突破为研究人员带来了许多新机遇,包括有机会更快地更好理解植物生物系统。本研究回顾了自2014年以来发表的52篇同行评议文章中与CRISPR系统在植物中的应用相关的农业应用。基于这一文献综述,CRISPR系统的主要用途是实现产量性能提高、生物强化、生物和非生物胁迫耐受性,水稻(Oryza sativa)是研究最多的作物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90a0/7288993/818efaa2bcc6/ETLS-1-169-g0001.jpg

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