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布氏锥虫中变异细胞表面糖蛋白基因1.8表达位点的稳定变异特异性转录本。

Stable variant-specific transcripts of the variant cell surface glycoprotein gene 1.8 expression site in Trypanosoma brucei.

作者信息

Shea C, Van der Ploeg L H

机构信息

Department of Genetics and Development, Columbia University, New York, New York 10032.

出版信息

Mol Cell Biol. 1988 Feb;8(2):854-9. doi: 10.1128/mcb.8.2.854-859.1988.

DOI:10.1128/mcb.8.2.854-859.1988
PMID:3352606
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC363216/
Abstract

The structure and transcriptional regulation of the 1.8 variant cell surface glycoprotein (VSG) gene expression site located on a 430-kilobase (kb) chromosome was examined in a 430-kb-chromosome-specific library. Using 32P-labeled nascent transcripts generated by nuclear run-on, we selected recombinant clones derived from the 430-kb chromosome which were coordinately activated with the 1.8 VSG gene. The results show that a repetitive region with a minimum size of 27 kb is coordinately activated with the 1.8 VSG gene. As with the 1.8 VSG gene, transcription is by RNA polymerases that are insensitive to the drug alpha-amanitin at concentrations up to 1 mg/ml. Transcription results in the generation of several stable variant-specific mRNAs. These mRNAs most likely belong to a family of repetitive expression-site-associated genes.

摘要

在一个430千碱基（kb）染色体特异性文库中，研究了位于430 kb染色体上的1.8变异型细胞表面糖蛋白（VSG）基因表达位点的结构和转录调控。利用核延伸产生的32P标记的新生转录本，我们筛选了来自430 kb染色体的重组克隆，这些克隆与1.8 VSG基因协同激活。结果表明，一个最小尺寸为27 kb的重复区域与1.8 VSG基因协同激活。与1.8 VSG基因一样，转录是由对浓度高达1 mg/ml的药物α-鹅膏蕈碱不敏感的RNA聚合酶进行的。转录产生了几种稳定的变异体特异性mRNA。这些mRNA很可能属于一个重复的表达位点相关基因家族。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/808b/363216/f2be25654b70/molcellb00062-0354-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/808b/363216/7e75388188bc/molcellb00062-0352-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/808b/363216/59c1964c8d6d/molcellb00062-0353-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/808b/363216/f2be25654b70/molcellb00062-0354-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/808b/363216/7e75388188bc/molcellb00062-0352-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/808b/363216/59c1964c8d6d/molcellb00062-0353-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/808b/363216/f2be25654b70/molcellb00062-0354-a.jpg

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引用本文的文献

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Structure and sequence of the gene for the largest subunit of trypanosomal RNA polymerase III.锥虫RNA聚合酶III最大亚基基因的结构与序列
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2
Alpha-amanitin resistant transcription of protein coding genes in insect and bloodstream form Trypanosoma brucei.
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3
RNA polymerase I can mediate expression of CAT and neo protein-coding genes in Trypanosoma brucei.RNA聚合酶I可介导布氏锥虫中CAT和新霉素蛋白编码基因的表达。

本文引用的文献

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