Cold Spring Harb Protoc. 2021 Feb 1;2021(2):2021/2/pdb.prot102178. doi: 10.1101/pdb.prot102178.
Recovery of intracellular proteins requires disruption of the host cell before the target protein is extracted and isolated. For cells enveloped in cell walls (such as ), vigorous methods are often required. This protocol focuses on lysis by sonication. Also included are methods for lysis by freeze-thaw and enzymatic treatments.
细胞内蛋白质的回收需要在提取和分离目标蛋白质之前破坏宿主细胞。对于被细胞壁包裹的细胞（如 ），通常需要使用剧烈的方法。本方案重点介绍超声裂解。还包括通过冻融和酶处理进行裂解的方法。
