Guo Lifeng, Li Zhiqi, Xu Xi, Pang Qiuying, Zhao Junwei
Key Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.
Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin 150040, PR China.
Int J Syst Evol Microbiol. 2019 Jun;71(3). doi: 10.1099/ijsem.0.004684. Epub 2021 Feb 2.
A novel actinobacterium, designated strain NEAU-D428, was isolated from rhizosphere soil of wheat and characterized using a polyphasic approach. Morphological and chemotaxonomic characteristics of the strain coincided with members of the genus . The 16S rRNA gene sequence analysis showed that the isolate was most closely related to NEAU-TX2-2 (99.2 %). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain clustered with CLES2 (99.1 %), but formed a separate subclade in the phylogenomic tree within the genus . The menaquinones were identified as MK-9(H), MK-9(H) and MK-9(H). The phospholipid profile was found to consist of diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, ninhydrin-positive glycophospholipid, phosphatidylinositol and phosphatidylinositol mannoside. The major fatty acids were identified as iso-C, C, 10-methyl C and C. Digital DNA-DNA hybridization and average nucleotide identity values between strain NEAU-D428 and NEAU-TX2-2, 2C-HV3, CLES2, ' Gxj-6 and NEAU-HEGS1-5 were 47.6 and 92.2 %, 47.5 and 92.2 %, 55.9 and 94.0 %, 33.1 and 86.8 %, and 33.6 and 87.1 %, respectively. These results and some physiological and biochemical properties demonstrated that the strain could be distinguished from its closest relatives. Therefore, it is proposed that strain NEAU-D428 should be classified as representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-D428 (=CGMCC 4.7523=DSM 109822).
从小麦根际土壤中分离出一株新型放线菌，命名为菌株NEAU-D428，并采用多相分类法对其进行了表征。该菌株的形态和化学分类特征与该属成员一致。16S rRNA基因序列分析表明，该分离株与NEAU-TX2-2关系最为密切（相似度为99.2%）。基于16S rRNA基因序列的系统发育分析表明，该菌株与CLES2聚类（相似度为99.1%），但在该属的系统发育树中形成了一个单独的亚分支。鉴定出的甲基萘醌为MK-9(H)、MK-9(H)和MK-9(H)。发现磷脂谱由双磷脂酰甘油、磷脂酰单甲基乙醇胺、磷脂酰乙醇胺、茚三酮阳性糖磷脂、磷脂酰肌醇和磷脂酰肌醇甘露糖苷组成。主要脂肪酸鉴定为异-C、C、10-甲基-C和C。菌株NEAU-D428与NEAU-TX2-2、2C-HV3、CLES2、'Gxj-6和NEAU-HEGS1-5之间的数字DNA-DNA杂交和平均核苷酸同一性值分别为47.6%和92.2%、47.5%和92.2%、55.9%和94.0%、33.1%和86.8%、33.6%和87.1%。这些结果以及一些生理生化特性表明，该菌株可以与其最密切的亲缘种区分开来。因此，建议将菌株NEAU-D428归类为该属一个新物种的代表，为此提出新物种名称sp. nov.。模式菌株为NEAU-D428(=CGMCC 4.7523=DSM 109822)。
