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利用牛津纳米孔长读测序技术构建 CanS41 菌株的高质量基因组资源

High-Quality Genome Resource of Strain CanS41 by Oxford Nanopore Long-Read Sequencing.

机构信息

State Key Laboratory of Crop Stress Biology of Arid Areas and NWAFU-Purdue Joint Research Center, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.

College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.

出版信息

Plant Dis. 2021 Aug;105(8):2231-2234. doi: 10.1094/PDIS-12-20-2615-A. Epub 2021 Sep 15.

DOI:10.1094/PDIS-12-20-2615-A
PMID:33529071
Abstract

is a necrotrophic mycoparasitic fungus with excellent biological control ability against numerous fungal plant pathogens. Here, we performed genomic sequencing of strain CanS41 using Oxford Nanopore sequencing technology. We generated a high-quality genome assembly (>99.99% accuracy), which comprised 26 contigs containing 60.68 Mb sequences with a GC content of 48.55% and a repeat content of 8.38%. The N contig length is 3.02 Mb. In total, 20,818 protein-coding genes were identified and functionally annotated. Genes encoding secreted proteins and carbohydrate-active enzymes as well as secondary metabolic gene clusters were also identified and analyzed. In summary, the high-quality genome assembly and gene annotation provided here will allow further exploration of biological functions and enhance biological control ability of .

摘要

是一种具有出色生物防治能力的坏死型真菌寄生菌,可有效防治多种真菌植物病原体。在此,我们利用 Oxford Nanopore 测序技术对 CanS41 菌株进行了基因组测序。我们生成了高质量的基因组组装体(>99.99%的准确率),其包含 26 个序列,总长 60.68Mb,GC 含量为 48.55%,重复序列含量为 8.38%。N 序列的长度为 3.02Mb。总共鉴定到 20,818 个编码蛋白的基因,并对其进行了功能注释。还鉴定和分析了编码分泌蛋白和碳水化合物活性酶以及次级代谢基因簇的基因。总之,这里提供的高质量基因组组装和基因注释将有助于进一步探索其生物学功能并提高其生物防治能力。

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