Department of Operative Dentistry and Periodontology, Martin-Luther-University Halle-Wittenberg, Germany.
Department of Operative Dentistry and Periodontology, Martin-Luther-University Halle-Wittenberg, Germany.
Int J Cardiol. 2021 May 15;331:255-261. doi: 10.1016/j.ijcard.2021.01.054. Epub 2021 Jan 30.
The composition of the subgingival microbiota is of great importance in both oral and systemic diseases. However, a possible association of the oral microbiome and cardiovascular (CV) outcome has not yet been considered in a complex model. The primary objective of the study (DRKS-ID: DRKS00015776) was to assess differences in complex subgingival bacterial composition, depending on the CV outcome in patients undergoing Coronary Artery Bypass Grafting Surgery (CABG).
We conducted a longitudinal cohort study enrolling 102 CV patients. After a one-year follow-up, the postoperative outcome was evaluated applying MACCE (Major Adverse Cardiac and Cerebrovascular Events) criteria. The complex oral microbiome was evaluated depending on CV outcome. The mathematical data processing included Qiime 2 software workflow and DADA2 pipeline as well as Human Oral Microbiome Database (HOMD) and Greengenes database classification. For identifying biomarkers distinguishing patients suffering from secondary CV events, the Cox Proportional Hazard Model for survival analysis was applied.
In total, 19,418 Operational Taxonomic Units (OTU) were mapped according to the HOMD and Greengenes database. No significant differences in alpha and beta diversity were linked to CV outcomes (Shannon index; Principal Coordinates Analysis). No biomarker predicting secondary CV events were identified applying the area under the receiver operating characteristic curve (AUC) model. However, in survival analysis, one biomarker of Saccharibacteria phylum (class: TM7-3, order: CW040, family: F16) was associated with the incidence of a secondary CV event (p = 0.016).
For the first time, a subgingival biomarker has been identified that supports a cardiovascular prognosis in CV patients undergoing coronary artery bypass grafting.
龈下微生物群的组成在口腔和全身疾病中都非常重要。然而，口腔微生物组与心血管（CV）结局之间的可能关联尚未在复杂模型中得到考虑。该研究的主要目的（DRKS-ID：DRKS00015776）是评估在接受冠状动脉旁路移植术（CABG）的患者中，根据 CV 结局，复杂龈下细菌组成的差异。
我们进行了一项纵向队列研究，纳入了 102 例 CV 患者。经过一年的随访，应用 MACCE（主要不良心脏和脑血管事件）标准评估术后结局。根据 CV 结局评估复杂的口腔微生物组。数学数据处理包括 Qiime 2 软件工作流程和 DADA2 管道以及人类口腔微生物组数据库（HOMD）和 Greengenes 数据库分类。为了识别区分继发 CV 事件患者的生物标志物，应用 Cox 比例风险模型进行生存分析。
根据 HOMD 和 Greengenes 数据库，共映射了 19,418 个操作分类单元（OTU）。CV 结局与 alpha 和 beta 多样性无显著差异（Shannon 指数；主坐标分析）。应用接受者操作特征曲线（AUC）模型未识别出预测继发 CV 事件的生物标志物。然而，在生存分析中，一个 Saccharibacteria 门的生物标志物（类：TM7-3，目：CW040，科：F16）与继发 CV 事件的发生率相关（p=0.016）。
这是首次确定了一个龈下生物标志物，该标志物支持接受冠状动脉旁路移植术的 CV 患者的心血管预后。
