Department of Respiratory and Critical Care Medicine.
Department of Otolaryngology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P. R. China.
Medicine (Baltimore). 2021 Jan 29;100(4):e22946. doi: 10.1097/MD.0000000000022946.
The present study aimed to investigate the correlation of long non-coding RNA nuclear-enriched abundant transcript 1 (lncRNA NEAT1) with microRNA (miR)-21, miR-124, and miR-125a, and their associations with disease risk, severity, and inflammatory cytokines of allergic rhinitis (AR).Totally 70 AR patients and 70 non-atopic obstructive snoring patients (as controls) were recruited. Inferior turbinate mucosa samples were collected from all participants for lncRNA NEAT1, its targets (miR-21, miR-124, and miR-125a), interleukin (IL)-4, IL-6, IL-10, and IL-17 detection via reverse transcription quantitative polymerase chain reaction. Disease severity of AR patients was assessed using individual nasal symptom score (INSS) and total nasal symptom score (TNSS).LncRNA NEAT1 was upregulated, while miR-21, miR-124, and miR-125a were downregulated in AR patients compared with controls. Additionally, lncRNA NEAT1, miR-21, and miR-125a displayed good values in differentiating AR patients from controls, while miR-124 could only slightly differentiate AR patients from controls. In AR patients, lncRNA NEAT1 was negatively associated with miR-21 and miR-125a, but not miR-124. However, in controls, no correlation of lncRNA NEAT1 with miR-21, miR-124, or miR-125a was observed. Furthermore, in AR patients, lncRNA NEAT1 was positively, while miR-21 and miR-125a was negatively associated with INSS (rhinorrhea, itching, congestion scores), TNSS and inflammatory cytokines; however, correlation of miR-124 with INSS, TNSS, and inflammatory cytokines was slight.LncRNA NEAT1 and its targets (miR-21 and miR-125a) present close correlations with disease risk, severity, and inflammation of AR, suggesting their potential as biomarkers for AR assessment.
本研究旨在探讨长链非编码 RNA 核丰富转录物 1（lncRNA NEAT1）与 microRNA（miR）-21、miR-124 和 miR-125a 的相关性及其与变应性鼻炎（AR）疾病风险、严重程度和炎症因子的关系。共招募了 70 例 AR 患者和 70 例非变应性阻塞性打鼾患者（作为对照组）。通过逆转录定量聚合酶链反应检测所有参与者的下鼻甲黏膜样本中的 lncRNA NEAT1、其靶标（miR-21、miR-124 和 miR-125a）、白细胞介素（IL）-4、IL-6、IL-10 和 IL-17。采用个体鼻症状评分（INSS）和总鼻症状评分（TNSS）评估 AR 患者的疾病严重程度。与对照组相比，AR 患者的 lncRNA NEAT1 上调，而 miR-21、miR-124 和 miR-125a 下调。此外，lncRNA NEAT1、miR-21 和 miR-125a 在区分 AR 患者与对照组方面具有良好的价值，而 miR-124 只能轻微地区分 AR 患者与对照组。在 AR 患者中，lncRNA NEAT1 与 miR-21 和 miR-125a 呈负相关，但与 miR-124 无关。然而，在对照组中，lncRNA NEAT1 与 miR-21、miR-124 或 miR-125a 无相关性。此外，在 AR 患者中，lncRNA NEAT1 与 INSS（流涕、瘙痒、充血评分）、TNSS 和炎症因子呈正相关，而 miR-21 和 miR-125a 呈负相关；然而，miR-124 与 INSS、TNSS 和炎症因子的相关性较弱。lncRNA NEAT1 及其靶标（miR-21 和 miR-125a）与 AR 的疾病风险、严重程度和炎症密切相关，提示其可能作为 AR 评估的生物标志物。
